UBE2V2 (Human) antibody

Overview


	Antigen:	UBE2V2/Mms2
	Antibody Type:	Polyclonal
	Application:	Western Blotting (WB)
	Reactivity:	Human
	Host:	Rabbit
	Quantity:	0.1 mg (0.5 mg/ml)
	Price:	280,00 € (Plus shipping costs and VAT)
	Order number:	ABIN123480
	Availability:	Ships in 3 to 10 days

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Additional Information: UBE2V2 (Human) antibody

Antigen	UBE2V2/Mms2
Immunogen	This antibody was generated by immunizing rabbit with a mixture of synthetic peptides containing amino acids 12-28 of human UBE2V2.
Reactivity	Human
Antibody Type	Polyclonal
Format	Purified
Host	Rabbit
Specificity	The differentiation and activation of monocytes (MO) and monocytic cells is modulated by 1alpha,25-dihydroxyvitamin D3 (Vitamin D3). Recently, using mRNA Differential Display technology, Fritsche J, et al have identified a gene which is induced in freshly isolated human blood MO that are cultured for 4 hours with Vitamin D3 (1). This cDNA encodes a putative 16.5 kD protein. The product of this gene has structural homology with yeast MMS2p and human CROC-1), which defines a structurally and functionally conserved Ubc-like protein family (2). This protein was called Ubiquitin E2 variant (UBE2V2) or MMS2 (methyl methanesulfonate sensitive 2). The yeast UEV protein, Mms2, is required together with Ubc13 for RAD6/RAD18-dependent postreplicative DNA repair in yeast (3). These proteins also play a central role in the assembly of K63-linked polyubiquitin chains (4,5) UEV/Ubc complexes have been implicated in the assembly of Lys63-linked polyubiquitin chains that act as a novel signal in postreplicative DNA repair and IkBa kinase activation. Recent crystal structure analysis provides direct evidence that the Mms2/Ubc13 heterodimer is necessary for DNA repair (6,7).

Application Details: UBE2V2 (Human) antibody

Application	Western Blotting (WB)
Application Notes	Western blot: 2.0 ug/ml. Recommended positive control: HL-60. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration	0.5 mg/ml
Buffer	This antibody is supplied as liquid, Protein G affinity purified IgG in PBS, pH 7.2, with 0.05% sodium azide as preservative and 0.2% gelatin as stabilizer.
Storage	Store the antibody at 4°C for one month or at –20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Restrictions	Research only

Publications: UBE2V2 (Human) antibody

Publications

Broomfield, Chow, Xiao:
"MMS2, encoding a ubiquitin-conjugating-enzyme-like protein, is a member of the yeast error-free postreplication repair pathway."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 95, Issue 10, pp. 5678-83, 1998/01 (PubMed)

Deng, Wang, Spencer et al.:
"Activation of the IkappaB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain."
In: Cell , Vol. 103, Issue 2, pp. 351-61, 2000/01 (PubMed)

VanDemark, Hofmann, Tsui et al.:
"Molecular insights into polyubiquitin chain assembly: crystal structure of the Mms2/Ubc13 heterodimer."
In: Cell , Vol. 105, Issue 6, pp. 711-20, 2001/01 (PubMed)

Michelin, Ruller, Ward et al.:
"Purification and biochemical characterization of a thermostable extracellular glucoamylase produced by the thermotolerant fungus Paecilomyces variotii."
In: Journal of industrial microbiology & biotechnology , Vol. 35, Issue 1, pp. 17-25, 2007/01 (PubMed)

Ulrich, Jentsch:
"Two RING finger proteins mediate cooperation between ubiquitin-conjugating enzymes in DNA repair."
In: The EMBO journal , Vol. 19, Issue 13, pp. 3388-97, 2000/01 (PubMed)

Images: UBE2V2 (Human) antibody

Detection of UBE2V2 inHL-60 cell lysate with anti-UBE2V2 pcAb (ABIN123480) at 2ug/ml dilution.

Image for UBE2V2 (Human) antibody (1)

External Information: UBE2V2 (Human) antibody

Google Scholar	Find more references for antigen UBE2V2/Mms2 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen UBE2V2/Mms2 (Bioinformatic Harvester (EMBL))