Ubiquitin (a.a. 50-65) antibody

Overview

Antigen:	Ubiquitin (a.a. 50-65)
Antibody Type:	Monoclonal
Application:	Enzyme Immunometric Assay (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
Host:	Mouse
Quantity:	100ul (~2mg/ml (Bradford & ELISA assa...)
Price:	754,00 € (Plus shipping costs and VAT)
Order number:	ABIN236621
Availability:	3-5 Business Days

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Additional Information: Ubiquitin (a.a. 50-65) antibody

Antigen	Ubiquitin (a.a. 50-65)
Antibody Type	Monoclonal
Format	Ascites, Liquid
Isotype	IgG1, k »Matching secondary antibodies
Description	MAb to Ubiquitin (a.a. 50-65) Monoclonal Antibody to Ubiquitin (amino acids 50-65)
Clone	Mrz 39
Host	Mouse
Specificity	Ubiquitin proteins. Residues 50-65 of ubiquitin. Reactive only to ubiquitinated protein, not to free ubiquitin. Highly reactive on formalin-fixed tissues. Can be used on native or detergent extracted tissues and the epitope is resistant to proteolytic enzyme treatment.

Application Details: Ubiquitin (a.a. 50-65) antibody

Application	Enzyme Immunometric Assay (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
Application Notes	ELISA (1:10,000 – 1:100,000) Western blot (1:1,000 – 1:100,000) Immunoprecipitation (1:10 – 1:100) Immunohistochemistry (1:100 – 1:10,000) Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Concentration	~2mg/ml (Bradford & ELISA assays)
Purification	Not applicable.
Buffer	Not applicable. Preservative: None
Storage	Best stored undiluted at –20°C to –70°C. Diluted antibody can be stored frozen or refrigerated in the presence of 0.1% sodium azide or other anti-bacterial agent. Avoid multiple freeze/thaw cycles..
Restrictions	For Research Use Only

Publications: Ubiquitin (a.a. 50-65) antibody

Publications

Galloway, Grundke-Iqbal, Iqbal et al.:
"Lewy bodies contain epitopes both shared and distinct from Alzheimer neurofibrillary tangles."
In: Journal of neuropathology and experimental neurology , Vol. 47, Issue 6, pp. 654-63, 1988/01 (PubMed)

Grundke-Iqbal, Wang, Iqbal et al.:
"Alzheimer paired helical filaments: identification of polypeptides with monoclonal antibodies."
In: Acta neuropathologica , Vol. 68, Issue 4, pp. 279-83, 1986/01 (PubMed)

Grundke-Iqbal, Iqbal, Quinlan et al.:
"Microtubule-associated protein tau. A component of Alzheimer paired helical filaments."
In: The Journal of biological chemistry , Vol. 261, Issue 13, pp. 6084-9, 1986/01 (PubMed)

Perry, Mulvihill, Fried et al.:
"Immunochemical properties of ubiquitin conjugates in the paired helical filaments of Alzheimer disease."
In: Journal of neurochemistry , Vol. 52, Issue 5, pp. 1523-8, 1989/01 (PubMed)

Wang, Grundke-Iqbal, Kascsak et al.:
"Alzheimer neurofibrillary tangles: monoclonal antibodies to inherent antigen(s)."
In: Acta neuropathologica , Vol. 62, Issue 4, pp. 268-75, 1984/01 (PubMed)

Wang, Iqbal, Bucht et al.:
"Alzheimer's disease: paired helical filament immunoreactivity in cerebrospinal fluid."
In: Acta neuropathologica , Vol. 82, Issue 1, pp. 6-12, 1991/01 (PubMed)

External Information: Ubiquitin (a.a. 50-65) antibody

Google Scholar	Find more references for clone Mrz 39 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Ubiquitin (a.a. 50-65) (Bioinformatic Harvester (EMBL))