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Product details for Ubiquitin E1 Conjugating Enzyme (Human) antibody
Ubiquitin E1 Conjugating Enzyme (Human) antibody
Overview
| Antigen: | Ubiquitin E1 Conjugating Enzyme |
| Antibody Type: | Polyclonal |
| Application: | ELISA, Western Blotting (WB), Immunoprecipitation (IP) |
| Reactivity: | Mouse (Murine), Human, Rat |
| Host: | Rabbit |
| Quantity: | |
| Price: | 287,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN129529 |
| Availability: | 7 days until shipment |
Additional Information: Ubiquitin E1 Conjugating Enzyme (Human) antibody
| Antigen | Ubiquitin E1 Conjugating Enzyme |
| Immunogen | . |
| Reactivity | Mouse (Murine), Human, Rat |
| Antibody Type | Polyclonal |
| Format | Liquid (sterile filtered) |
| Host | Rabbit |
Application Details: Ubiquitin E1 Conjugating Enzyme (Human) antibody
| Application | ELISA, Western Blotting (WB), Immunoprecipitation (IP) |
| Concentration | 5.0 mg/ml (by UV absorbance at 280 nm) |
| Purity | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
| Purification | This purified antibody is directed againsthuman Ubiquitin Activating Enzyme E1 protein. The product waspurified from monospecific antiserum by a multi step procedure. ABLAST analysis was used to suggest that this antibody wouldreact with Ubiquitin Activating Enzyme E1 protein from human(100%) rabbit (96%), mouse (95%), rat (95%) and dog (93%)based on a high degree of sequence homology. Cross reactivityagainst this protein from other sources has not been determined.Relevant Link(s):Swis-Prot P22314NCBI Link NP_003325 ELISA primary antibody diluted to 1:1,000. Incubationwas for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution ofIRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L]MX10 (611-132-122) for 45 min at roomtemperature. Molecular weight markers are shownin lane 2 (700 nm channel). IRDyeTM800fluorescence image was captured using theOdyssey® Infrared Imaging System developed byLI-COR. IRDye is a trademark of LI-COR, Inc.Other detection systems will yield similar results.12Figure 2. Immunohistochemistry. Affinity Purifiedanti-Ubiquitin Activating Enzyme antibody was used at a 10µg/ml to detect UBE1 in a variety of tissues including adrenal,breast, colon (epithelium), kidney, liver, lung (respiratoryepithelium), ovary (oocyte and endothelium), pancreas (isletand exocrine), placenta, prostate (epithelium), skin(epithelium), spleen (lymphocytes), stomach (chief), testis,thymus, tonsil, and uterus (glandular, stroma). In many cells apunctate nuclear staining was observed. Other cells showedboth cytoplasmic and nuclear staining. This image showsUBE1 staining of human lung tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communi-cation, TinaRoush, LifeSpanBiosciences, Seattle, WA. Protein Sequence: Human Ubiquitin Activating Enzyme E1, 1058 aa, predicted MW 117.8 kDa |
| Storage | Store vial at 4° C prior to restoration.Reconstitute with 0.1 ml of deionized water or equivalent. Forextended storage aliquot contents and freeze at -20° C orbelow. Avoid cycles of freezing and thawing. Expiration date isone (1) year from date of restoration. |
| Research Area | Proteases, Amino Acids |
| Restrictions | For Research Use only |
External Information: Ubiquitin E1 Conjugating Enzyme (Human) antibody
| Google Scholar | Find more references for antigen Ubiquitin E1 Conjugating Enzyme on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Ubiquitin E1 Conjugating Enzyme (Bioinformatic Harvester (EMBL)) |
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