ZAP70 (phospho Y292) (Human) antibody

Overview

Antigen	ZAP70 (phospho Y292)
Immunogen	Synthetic peptide derived from a region of human ZAP70 that contains tyrosine 292 (unfortunately, the amino acid sequence is considered to be commercially sensitive)
Reactivity	Human, Mouse (Murine)
Antibody Type	Polyclonal
Format	Liquid
Isotype	IgG »Matching secondary antibodies
Description	ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling. Phosphorylation of tyrosines 315 and 319 plays an important role in mediating the positive and negative selection of T cells in thymus.
Host	Rabbit

Application	Western Blotting (WB)
Application Notes	WB: Use at a dilution of 1/1000. Predicted molecular weight: 74.3 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Purity	Immunogen affinity purified
Buffer	Preservative: 0.05% Sodium AzideConstituents: Dulbeccos PBS without magnesium and calcium, 50% Glycerol, PBS, 1mg/ml BSA, pH 7.3
Restrictions	For research use only

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