ZBP-89 (Human) antibody

Overview


	Antigen:	ZBP-89
	Antibody Type:	Polyclonal
	Application:	Enzyme Immunoassay (EIA), Western Blotting (WB)
	Reactivity:	Human
	Host:	Rabbit
	Quantity:	0.1 ml (90 mg/ml (by Refractometry))
	Price:	340,00 € (Plus shipping costs and VAT)
	Order number:	ABIN117932
	Availability:	Ships in 3 to 10 days

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Additional Information: ZBP-89 (Human) antibody

Antigen	ZBP-89
Immunogen	Purified full length ZBP-89 recombinant protein expressed in E.coli. Label: Horseradish Peroxidase (HRP)
Reactivity	Human
Antibody Type	Polyclonal
Format	Serum
Host	Rabbit
Specificity	This product was prepared from monospecific antiserum by delipidation and defibrination. This polyclonal antibody is specific for human ZBP-89. Reactivity with ZBP-89 from other species has not been determined. The GI tract abundantly expresses growth factors many of which bind and activate the EGF receptor present on mucosal cells. One such factor is the zinc finger protein (ZBP-89) that binds to a GC-rich DNA element in the gastrin promoter and confers EGF responsiveness. The fulllength protein functions as a repressor of growth factor signals regulating the gastrin promoter. Several other growth related promoters are also regulated by ZBP-89. ZBP-89 is one of a family of related transcriptional regulators. It has been reported in recent studies that ZBP-89 regulates growth in part by stimulating the cyclin-dependent kinase inhibitor, p21waf1, in a butyrate-dependent manner through recruitment of the histone acetyl transferase p300. Moreover, ZBP-89 triggers growth arrest in a p53-dependent manner by preventing nuclear export of p53. ZBP-89 also induces apoptosis, but this process occurs independent of p53. Sequence (Human): 1 mniddklegl flkcggidem qssrtmvvmg gvsgqstvsg elqdsvlqdr smphqeilaa 61 devlqesemr qqdmishdel mvheetvknd eeqmetherl pqglqyalnv pisvkqeitf 121 tdvseqlmrd kkqirepvdl qkkkkrkqrs pakiltined gslglktpks hvcehcnaaf 181 rtnyhlqrhv fihtgekpfq csqcdmrfiq kyllqrheki htgekpfrcd ecgmrfiqky 241 hmerhkrths gekpyqceyc lqyfsrtdrv lkhkrmchen hdkklntcai kgglltseed 301 sgfstspkdn slpkkkrqkt ekkssgmdke saldksdlkk dkndylpvys sstkvkdeym 361 vaeyavemph ssvggshled asgeihppkl vlkkinskrs lkqpleqnqt isplstyees 421 kvskyafelv dkqalldseg nadidqvdnl qegpskpvhs stnyddamqf lkkkrylqaa 481 snnsreyaln vgtirsqpsv tqaavasvid esttasiles qalnveiksn hdknvipdev 541 lqtlldhysh kangqheisf svadtevtss isinssevpe vtpsenvgss sqasssdkan 601 mlqeyskflq qaldrtsqnd aylnspslnf vtdnqtlpnq pafssidkqv yatmpinsfr 661 sgmnsplrtt pdkshfgliv gdsqhsfpfs gdetnhasat stqdfldqvt sqkkaeaqpv 721 hqayqmssfe qpfrapyhgs ragiatqfst angqvnlrgp gtsaefsefp lvnvndnrag 781 mtsspdattg qtfg

Application Details: ZBP-89 (Human) antibody

Application	Enzyme Immunoassay (EIA), Western Blotting (WB)
Application Notes	This polyclonal antibody reacts human ZBP-89 in a variety of immunological assays including western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. For immunoblotting a 1 :5,000 s dilution is recommended. A band at approximately 89 kDa corresponding to human ZBP-89 is detected. Human monocytes or macrophages or nuclear extracts from PMA treated U937 cellcan be used as a positive control. For ELISA a 1:10,000 to 1:30,000 dilution is recommended. Researchers should determine optimal titers for other applications. Human monocytes or macrophages or nuclear extracts from PMA treated U937 cells can be used as a positive control.
Concentration	90 mg/ml (by Refractometry)
Buffer	Liquid (sterile filtered) , 0.1% (w/v) Sodium Azide as preservative.
Storage	Store vial at -20° C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.
Restrictions	Research only

Publications: ZBP-89 (Human) antibody

Publications

Bai, Merchant:
"Transcription factor ZBP-89 is required for STAT1 constitutive expression."
In: Nucleic acids research , Vol. 31, Issue 24, pp. 7264-70, 2003/01 (PubMed)

Bai, Merchant:
"Transcription factor ZBP-89 cooperates with histone acetyltransferase p300 during butyrate activation of p21waf1 transcription in human cells."
In: The Journal of biological chemistry , Vol. 275, Issue 39, pp. 30725-33, 2000/01 (PubMed)

Bai, Merchant:
"ZBP-89 promotes growth arrest through stabilization of p53."
In: Molecular and cellular biology , Vol. 21, Issue 14, pp. 4670-83, 2001/01 (PubMed)

Chen, Merchant, Lai et al.:
"Mutation of p53 in recurrent hepatocellular carcinoma and its association with the expression of ZBP-89."
In: The American journal of pathology , Vol. 162, Issue 6, pp. 1823-9, 2003/01 (PubMed)

Dawson, Park, Chen et al.:
"Retinoic acid (RA) receptor transcriptional activation correlates with inhibition of 12-O-tetradecanoylphorbol-13-acetate-induced ornithine decarboxylase (ODC) activity by retinoids: a potential role for trans-RA-induced ZBP-89 in ODC inhibition."
In: International journal of cancer. Journal international du cancer , Vol. 91, Issue 1, pp. 8-21, 2001/01 (PubMed)

Wieczorek, Lin, Perkins et al.:
"The zinc finger repressor, ZBP-89, binds to the silencer element of the human vimentin gene and complexes with the transcriptional activator, Sp1."
In: The Journal of biological chemistry , Vol. 275, Issue 17, pp. 12879-88, 2000/01 (PubMed)

Images: ZBP-89 (Human) antibody

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External Information: ZBP-89 (Human) antibody

Google Scholar	Find more references for antigen ZBP-89 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen ZBP-89 (Bioinformatic Harvester (EMBL))