KIAA0191 (Human) antibody

Overview

Antigen	ZCCHC11 / PAPD3 / KIAA0191
Gene ID	23318
Immunogen	Peptide with sequence RERCPHPPRGNVSE, from the C Terminus of the protein sequence according to NP_056084.1, NP_001009882.1, NP_001009881.1
Reactivity	Human
Antibody Type	Polyclonal
Format	Aff - Purified
Description	Official Gene Symbol: ZCCHC11 Accession Number(s): NP_056084.1, NP_001009882.1, NP_001009881.1 Gene Ontology terms: nucleic acid binding This antibody is expected to recognize all 3 reported isoforms (as represented by NP_056084.1, NP_001009882.1, NP_001009881.1). . Principal Names: KIAA0191, zinc finger, CCHC domain containing 11, PAPD3.
Host	Goat
Specificity	Expected reactivity from sequence similarity: Human, Mouse, Rat

Application	Western Blotting (WB)
Application Notes	Peptide ELISA: (antibody detection limit dilution 1:32,000).Western blot: Approx 250-300 kDa and 30-35 kDa band seen in Raji Lysate. Recommended for use at 1-3 ug/ml. Please note that the band we consistently detect in western blots differs from the predicted size of 181kDa. Currently we cannot find an explanation for this in the literature. However, it has several consensus glycosylation sites. Glycosylations are known to affect migration of proteins on a SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press].
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer	0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage	Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions	For Research Use Only

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EMBL Harvester	Search human, mouse or rat genome for antigen ZCCHC11 / PAPD3 / KIAA0191 (Bioinformatic Harvester (EMBL))