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Product details for alpha COP I (Human) antibody
alpha COP I (Human) antibody
Overview
| Antigen: | Alpha COP I |
| Antibody Type: | Polyclonal |
| Application: | Immunocytochemistry (ICC), Western Blotting (WB) |
| Reactivity: | Human, Rat |
| Host: | Rabbit |
| Quantity: | |
| Price: | 388,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN148334 |
| Availability: | 7 to 10 days until shipment |
Additional Information: alpha COP I (Human) antibody
| Antigen | Alpha COP I |
| Immunogen | Synthetic peptide: MLTKFETKSARVKGLSFHP-C, corresponding to amino acids 1-19 of Rat alpha COP I. |
| Reactivity | Human, Rat |
| Antibody Type | Polyclonal |
| Format | Liquid |
| Isotype | IgG »Matching secondary antibodies |
| Description | Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COPI) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COPI, and ARF. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits. |
| Host | Rabbit |
| Specificity | Detects coatomer-protein I alpha (COP I alpha). |
Application Details: alpha COP I (Human) antibody
| Application | Immunocytochemistry (ICC), Western Blotting (WB) |
| Application Notes | Immunocytochemistry: use at 3 ug/ml. Western Blot: use at 1 ug/ml. By Western blot, this antibody detects an ~140 kDa protein representing COPI alpha from PC-12 cell extract. This antibody also detects an unidentified protein at ~100 kDa. Immunocytochemical staining of alpha COP I using ABIN148334 results in alpha COP localization to the Golgi complex and to distinct vesicular structures scattered throughout the cytoplasm. |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Buffer | PBS, 1 mg/ml BSA, 0.05% sodium azide |
| Storage | Store at 4 C. Aliquot and store at -20 C long-term. Avoid freeze-thaw cycles. |
| Restrictions | For research use only |
External Information: alpha COP I (Human) antibody
| Google Scholar | Find more references for antigen alpha COP I on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen alpha COP I (Bioinformatic Harvester (EMBL)) |
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