b-ARK1 (Human) antibody

Overview

Antigen	B-ARK1
Immunogen	Purified recombinant human betaARK1
Reactivity	Human, Rat
Antibody Type	Monoclonal
Format	Liquid
Isotype	IgG1 »Matching secondary antibodies
Description	The beta-adrenergic receptor kinase 1 (betaARK1) was originally isolated as a ser/thr kinase that phosphorylates cardiac beta-adrenergic receptors. It has subsequently been found to phosphorylate many other kinds of G protein coupled receptors (GPCR), as well. It is now recognized as one of a family of G protein coupled receptor kinases that are important in regulating the activity of GPCRs. Phosphorylation of GCPRs by betaARK1, and other related kinases, generally occurs only after the receptor has been activated by ligand binding. The phosphorylation results in the uncoupling of the GPCR from G proteins by binding to beta-arrestin. The binding of beta-arrestin may also result in receptor internalization, mediated by clatherin and dynamin and/or the activation of the MAP kinase cascade. ß,ARK1 is found in many cell types and may also have a role in the regulation of the microtubular cytoskeleton. The levels of ß,ARK1 enzymatic activity, protein and mRNA are all elevat
Clone	5D5
Host	Mouse
Specificity	This antibody is specific for betaARK1.

Application Notes	For WB: Use at a dilution of 1:100. For IHC: Use at a diltuion of 1:100. This antibody specifically recognizes purified recombinant ß,ARK1 on immunoblots. It is particularly effective for the examination of the immunocytochemical analysis of the expression of ß,ARK1 in cells and tissue. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Purity	Tissue culture supernatant
Buffer	N/A
Storage	Keep as concentrated solution. Store at 4?C short term. For extended storage aliquot and store at -20?C or below. Avoid freeze-thaw cycles.
Restrictions	For research use only

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EMBL Harvester	Search human, mouse or rat genome for antigen b-ARK1 (Bioinformatic Harvester (EMBL))