Product details for  c-erbB-2 (Dog (Canine)) antibody
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c-erbB-2 (Dog (Canine)) antibody

Overview

Antigen: C-erbB-2 / Her-2 / neu (1240-1260)
Antibody Type: Monoclonal
Application: Enzyme Immunoassay (EIA), Western Blotting (WB), Immunoblotting (IB)
Reactivity: Dog (Canine), Human, Mouse (Murine)
Host: Mouse
Quantity: 0.1 mg
Price: 330,00 €   (Plus shipping costs and VAT)
Order number: ABIN126791
Availability: 3 to 10 days until shipment
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Additional Information:  c-erbB-2 (Dog (Canine)) antibody

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Antigen C-erbB-2 / Her-2 / neu (1240-1260)
Immunogen Peptide conjugated to KLH.
Reactivity Dog (Canine), Human, Mouse (Murine)
Antibody Type Monoclonal
Format Purified
Isotype IgG1IgG1 »Matching secondary antibodies
Description Molecular weight of antigen is 185kDa.
Clone 19D2
Host Mouse
Specificity ErbB2 is a member of the EGFR/erbB-receptor tyrosine kinase family. Dysregulation of erbB2 and/or activation of do wnstream signaling pathways has been implicated in many human cancers. ErbB2 is activated upon ligand dependent heterodimerization with EGFR, erbB3 or erbB4. ErbB2 homodimers are not favored due to the lack of an erbB2 specific extracellular ligand. Heterodimerization with EGFR or erbB4 leads to activation of the intrinsic tyrosine kinase activity of EGFR or erbB4 resulting in phosphorylation of multiple tyrosine residues within the erbB2 intracellular domain: Tyr 1023, Tyr 1112, Tyr 1139, Tyr 1196, Tyr 1222, and Tyr 1248. This antibody specifically recognizes erbB2 at 185 kDa. Epitope: aa 1240 - 1260. Reacts with human, mouse and dog.

Application Details:  c-erbB-2 (Dog (Canine)) antibody

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Application Enzyme Immunoassay (EIA), Western Blotting (WB), Immunoblotting (IB)
Application Notes Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% (v/v) Tween 20, 2x PBS. ELISA: 0.1 µg/ml. Positive control: SKOV-3, untreated. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Format Purified
Buffer This purified antibody lyophilized from 1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 1 ml H2O (15 min, RT).
Storage For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen,,, reconstituted antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freeze-thaw cycles. Shelf life: one year from despatch.
Research Area Receptors, Growth Factors
Restrictions For Research Use only

External Information:  c-erbB-2 (Dog (Canine)) antibody

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Google Scholar Find more references for clone 19D2 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen c-erbB-2 / Her-2 / neu (1240-1260) (Bioinformatic Harvester (EMBL))