Product details for  c-erbB-2 (Dog (Canine)) antibody
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c-erbB-2 (Dog (Canine)) antibody

Overview

Antigen: C-erbB-2 / Her-2 / neu pTyr1112
Antibody Type: Monoclonal
Application: Enzyme Immunoassay (EIA), Western Blotting (WB), Immunoblotting (IB)
Reactivity: Dog (Canine), Human, Mouse (Murine), Rat
Host: Mouse
Quantity: 0.1 mg
Price: 330,00 €   (Plus shipping costs and VAT)
Order number: ABIN126789
Availability: 3 to 10 days until shipment
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Additional Information:  c-erbB-2 (Dog (Canine)) antibody

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Antigen C-erbB-2 / Her-2 / neu pTyr1112
Immunogen Synthetic phosphopetide conjugated to KLH.
Reactivity Dog (Canine), Human, Mouse (Murine), Rat
Antibody Type Monoclonal
Format Purified
Isotype IgG1IgG1 »Matching secondary antibodies
Description Molecular weight of antigen is 185kDa.
Clone 19G5
Host Mouse
Specificity ErbB2 is a member of the EGFR/erbB-receptor tyrosine kinase family. Dysregulation of erbB2 and/or activation of do wnstream signaling pathways has been implicated in many human cancers.ErbB2 is activated upon ligand dependent heterodimerizatio n with EGFR, erbB3 or erbB4. ErbB2 homodimers are not favored due to the lack of an erbB2 specific extracellular ligand. Heterodimerization with EGFR or erbB4 leads to activation of the intrinsic tyrosine kinase activity of EGFR or erbB4 resulting in phosphorylation of multiple tyrosine residues within the erbB2 intracellular domain: Tyr 1023, Tyr 1112, Tyr 1139, Tyr 1196, Tyr 1222, and Tyr 1248.Transphosphorylation via src family kinases leads to phosphorylation of Tyr 877, via PKC of Thr 669, via CamKinase2 of Ser 1113. Phosphorylation of Thr 669 and Ser 1113 interferes with erbB2 endocytosis and degradation This antibody specifically recognizes erbB2 phosphorylated at tyrosine 1112 at 185 kDa. Epitope: phospho-Tyr 1112. Reacts with human, mouse, rat and dog.

Application Details:  c-erbB-2 (Dog (Canine)) antibody

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Application Enzyme Immunoassay (EIA), Western Blotting (WB), Immunoblotting (IB)
Application Notes Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% (v/v) Tween 20, 2x PBS. ELISA: 0.05 µg/ml. Positive control: SKOV-3, EGF-treated. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Format Purified
Buffer This purified antibody lyophilized from 1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 1 ml H2O (15 min, RT).
Storage Aliquote and freeze in liquid nitrogen,,, reconstituted antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freeze-thaw cycles. Shelf life: one year from despatch.
Research Area Receptors, Growth Factors, Phospho-specific antibodies
Restrictions For Research Use only

External Information:  c-erbB-2 (Dog (Canine)) antibody

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Google Scholar Find more references for clone 19G5 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen c-erbB-2 / Her-2 / neu pTyr1112 (Bioinformatic Harvester (EMBL))