Product details for  c-fos pSer374 (Dog (Canine)) antibody
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c-fos pSer374 (Dog (Canine)) antibody

Overview

Antigen: C-fos pSer374 (incl. pos control)
Antibody Type: Monoclonal
Application: Enzyme Immunoassay (EIA), Western Blotting (WB), Immunoblotting (IB)
Reactivity: Dog (Canine), Human
Host: Mouse
Quantity: 0.1 mg
Price: 330,00 €   (Plus shipping costs and VAT)
Order number: ABIN126800
Availability: 3 to 10 days until shipment
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Additional Information:  c-fos pSer374 (Dog (Canine)) antibody

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Antigen C-fos pSer374 (incl. pos control)
Immunogen Synthetic phosphopeptide conjugated to KLH.
Reactivity Dog (Canine), Human
Antibody Type Monoclonal
Format Purified
Isotype IgG1IgG1 »Matching secondary antibodies
Description Molecular weight of antigen is 50kDa.
Clone 3-40E-005
Host Mouse
Specificity The immediated early gene product c-Fos is expressed following mitogenic stimulation. c-Fos functions as a sensor for MAPK signal duration. When MAPK activation is transient, MAPK activity declines before accumulation of the c-Fos protein. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at serine 374. Phosphorylation stabilizes the Fos protein and primes c-Fos for additional phosphorylation at threonine 325. This antibody specifically interacts with c-Fos phosphorylated at serine 374 and does not crossreact with the non-phosphorylated c-Fos nor with unrelated serine-phosphorylated proteins. The antibody is suitable for Western blot and ELISA applications. Epitope: S L S pS P T L (phosphoserine 374). Reacts with human and dog.

Application Details:  c-fos pSer374 (Dog (Canine)) antibody

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Application Enzyme Immunoassay (EIA), Western Blotting (WB), Immunoblotting (IB)
Application Notes Western Blot: 0.5 µg/ml for HRPO/ECL detection Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% (v/v) Tween 20, 2x PBS. ELISA: 0.1 µg/ml (protein ELISA),,, capture ELISA: not determined. Positive control: HepG2 EGF-treated. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Format Purified
Buffer This purified antibody lyophilized from 1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 1 ml H2O (15 min, RT).
Storage For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen,,, reconstituted antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freeze-thaw cycles. Shelf life: one year from despatch.
Restrictions For Research Use only

External Information:  c-fos pSer374 (Dog (Canine)) antibody

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Google Scholar Find more references for clone 3-40E-005 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen c-fos pSer374 (incl. pos control) (Bioinformatic Harvester (EMBL))