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Product details for p13 (Human) antibody
p13 (Human) antibody
Overview
| Antigen: | P13 (Myeloid Cells) |
| Antibody Type: | Monoclonal |
| Application: | Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)) |
| Reactivity: | Human |
| Host: | Mouse |
| Quantity: | |
| Price: | 340,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN116935 |
| Availability: | Ships in 3 to 10 days |
Additional Information: p13 (Human) antibody
| Antigen | P13 (Myeloid Cells) |
| Immunogen | A BALB/c mouse was immunized with nuclei from Pokeweed mitogen stimulated human peripheral blood lymphocytes. Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse cells using conventional hybridoma technology. |
| Reactivity | Human |
| Antibody Type | Monoclonal |
| Format | Purified |
| Isotype | IgG1 »Matching secondary antibodies |
| Clone | BM3 |
| Host | Mouse |
| Specificity | This protein A purified mouse monoclonal antibody reacts specifically with p13 in human granulocytes and monocytes residing in lymphoid and non-lymphoid tissues The antibody recognizes a 13 kDa band corresponding to p13. Cross reactivity from other sources has not been determined. |
Application Details: p13 (Human) antibody
| Application | Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)) |
| Application Notes | This antibody is suitable for flow cytometry and frozen section immunohistochemistry. The antibody does not stain tissue after paraffin treatment. For immunohistochemistry dilute the antibody 1:100. Researchers should determine optimal titers for other applications. Other applications not tested. Background Information: p13 is a specific marker for human myeloid cells. Induction studies using HL-60 cells show that p13 is expressed during the early phases of myeloid differentiation. The mAb anti-p13 recognizes a nuclear antigen expressed in human granulocytes (98%) and monocytes (80%) residing in lymphoid and non-lymphoid tissues. BM3 is an early marker of myeloid differentiation. Immunoprecipitation experiments using S35 methionine labeled human myeloid leukemia cells show that BM3 identifies a 13,000 Dalton protein. |
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Buffer | This antibody is supplied as liquid (sterile filtered), Protein A affinity purified immunoglobulin fraction in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide as preservative. |
| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. 1 |
| Restrictions | Research only |
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