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Product details for Thymine Dimer antibody
Thymine Dimer antibody
Overview
| Antigen: | Thymine Dimer |
| Antibody Type: | Monoclonal |
| Application: | Blocking Antibody, Dot Blot (Dot), Southern Blotting (SB), Western Blotting (WB) |
| Host: | Mouse |
| Quantity: | |
| Price: | 247,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN145119 |
| Availability: | 7 to 10 days until shipment |
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Additional Information: Thymine Dimer antibody
| Antigen | Thymine Dimer |
| Immunogen | Chemical / Small Molecule. |
| Antibody Type | Monoclonal |
| Format | Liquid |
| Isotype | IgG1 »Matching secondary antibodies |
| Description | Non-radioactive labeling of DNA is typically based on the enzymatic incorporation of modified nucleotides, carrying a small chemical moiety such as biotin, digoxigenin or fluorescein. These tags are subsequently detected by specific reagents such as streptavidin or a specific antibody coupled to a signal-producing enzyme. Although very efficient and reliable, labeling by in vitro polymerization is time-consuming, expensive, and may require various post-label purification steps to remove an excess of unincorporated precursors. An alternative strategy for DNA labeling, is based on the UV-induced formation of cyclobutane thymine dimers. Several methods have been described for the detection of thymine dimers, which are based on chromato-graphic analysis, and on biochemical analysis with endonucleases specific for UV-irradiated DNA. In addition, methods utilizing antibodies specific for pyrimidine dimers and other UV-induced DNA lesions have evolved, which permit the study of the induct |
| Clone | H3 |
| Host | Mouse |
| Specificity | Monoclonal Anti-Thymine Dimer reacts specifically with the (5?-6?) cyclobutane type of homothymine or thymine-cytosine heterodimers. The antibody reacts with thymine dimers in single-stranded DNA, and has a lower affinity for the dimer in short oligonucle |
Application Details: Thymine Dimer antibody
| Application | Blocking Antibody, Dot Blot (Dot), Southern Blotting (SB), Western Blotting (WB) |
| Application Notes | SB: Use at a concentration of 0.5-1.0 ug/ml. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Format | Liquid |
| Concentration | 2 mg/ml |
| Purity | Protein G purified |
| Buffer | Preservative: 15mM Sodium AzideConstituents: PBS. pH 7.4 |
| Restrictions | For research use only |
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