CD26 (Human) antibody

Overview


	Antigen:	CD26
	Antibody Type:	Monoclonal
	Application:	Flow Cytometry (FACS), Immunohistochemistry (IHC)
	Reactivity:	Human
	Host:	Mouse, Rat
	Quantity:	6 ml
	Price:	151,00 € (Plus shipping costs and VAT)
	Order number:	ABIN130568
	Availability:	7 to 10 days until shipment

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Additional Information: CD26 (Human) antibody

Antigen	CD26
Immunogen	This product is culture supernatant (RPMI 1640 medium containing 10% fetal calf serum and 0.1% NaN3) of hybridoma established by fusion of mouse myeloma P3-U1 with Balb/c splenocyte immunized with human placental dipeptidyl aminopeptidase IV. *Azide may react with copper or lead in plumbing systems to form explosive metal azides. Therefore, always flush drains thoroughly with plenty of water when disposing of materials containing azide.
Reactivity	Human
Antibody Type	Monoclonal
Format	SN
Isotype	IgG1 »Matching secondary antibodies
Description	CD26 is a widely distributed 110 kDa membrane-bound glycoprotein that has a dipeptidyl peptidase IV (DPP IV) activity in its extracellular domain. This molecule is expressed in wide variety of normal tissues and cell populations including lymphocytes. On T cells, its expression is preferentially restricted to the CD4 + helper/memory population, and CD26 can deliver a potent T cell co-stimulatory activation signal. It is also reported that thyroid carcinoma expresses aberrant CD26 activity and that enzyme-histochemical staining of CD26 is very useful for pathological diagnosis of thyroid tumors.
Clone	44-4
Host	Mouse, Rat
Specificity	This antibody detects human CD26 (DPP IV) antigen on flow cytometry and immunohistochemistry.

Application Details: CD26 (Human) antibody

Application	Flow Cytometry (FACS), Immunohistochemistry (IHC)
Application Notes	Flow cytometric analysis for lymphocytes 1) Preparation of the lymphocytes from whole blood as below. 1. Prepare 5 ml of whole blood pretreated with EDTA. 2. Add 1. onto the 10 ml of Ficoll-paque 3. Centrifuge at 1600 rpm for 30 min. at room temperature. 4. Remove supernatant and collect the Immunohistochemical detection of human CD26 on paraffin embedded section of human Thyroid carcinoma. lymphocytes’ layer by careful pipetting. 5. Add appropriate volume of PBS and mix gently. 6. Centrifuge at 1600 rpm for 8 min. at 4°C. 7. Remove supernatant and mix the cells by gentle tapping, then add appropriate volume of PBS. 8. Centrifuge at 1000 rpm for 8 min. at 4°C. 9. Repeat steps 7. and 8. three times. 2) Prepare the indicated solutions as below. Wash solution: 2% FCS/0.1%NaN3 in PBS 1st antibody solution: anti-Human CD26 (DPP IV) antibody (ready to use) 2nd antibody solution: Anti-Mouse IgG conjugated with FITC (IMMUNOTECH: Cat No. sections: ABC method 1) Deparaffinize section, hydrate to PBS. (Xylene 3 times, Ethanol 3 times, PBS 3 times, immerse the section for 3-5 minutes each.) 2) Remove slides from PBS and cover each section with 100-200 µl of 3% H2O2 for 10 min. at room temperature to block endogenous peroxidase activity. Do not treat frozen section with 3% H2O2. Wash twice in PBS for 5 min. each. 3) Remove slides from PBS, wipe gently around each section and cover tissues with 100-200 µl of Protein Blocking Agent (Ultratech HRP Kit, IMMUNOTECH Cat# IM-2391) for 5min. to block non specific antibody staining. Do not wash. 4) Tip off the blocking buffer, wipe gently around each section and cover tissues with 100-200 µl of primary antibody solution at the concentration of as suggested in Applications. 5) Incubate the section for 1 hr. at room temperature (or 37°C for 10 min.). 6) Wash the slide twice in PBS for 5 min. each. 7) Wipe gently around each section and cover tissues with 100-200 µl of Polyvalent Biotinylated antibody (Ultratech HRP Kit). 8) Incubate for 30 min. at room temperature. 9) Wash as in 6). 10) Wipe gently around each section and cover tissues with 100-200 µl of Streptavidin-Peroxidase (Ultratech HRP Kit) 11) Incubate for 30 min. at room temperature. 12) Wash as in 6). 13) Visualize with DAB substrate/chromogen (Ultratech DAB, IMMUNOTECH Cat# IM-2394) for 5 min. *DAB is a suspected carcinogen and must be handled with care. Always wear gloves. 14) Wash as in 6). 15) Counterstain in hematoxylin for 2min. 16) Wash as in 6). 17) Dehydrate by immersing in Ethanol 3 times for 3 min. each followed by immersing in Xylene twice for 3min. each. 18) Mounting. 050630-1 Immunohistochemistry: ready-to-use. Flow Cytometry: ready-to-use
Research Area	CD Antigens
Restrictions	For Research Use Only

Publications: CD26 (Human) antibody

Publications

Kotani, Kawano, Suganuma et al.:
"Immunohistochemical localization of dipeptidyl aminopeptidase IV in thyroid papillary carcinoma."
In: International journal of experimental pathology , Vol. 73, Issue 2, pp. 215-22, 1992/01 (PubMed)

Kotani, Asada, Aratake et al.:
"Diagnostic usefulness of dipeptidyl aminopeptidase IV monoclonal antibody in paraffin-embedded thyroid follicular tumours."
In: The Journal of pathology , Vol. 168, Issue 1, pp. 41-5, 1993/01 (PubMed)

Images: CD26 (Human) antibody

Immunohistochemical detection of human CD26 on paraffin embedded section of human Thyroid carcinoma.

Image for CD26 (Human) antibody (1)

FACS

Image for CD26 (Human) antibody (2)

External Information: CD26 (Human) antibody

Google Scholar	Find more references for clone 44-4 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen CD26 (Bioinformatic Harvester (EMBL))