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Product details for TNF alpha (Human) antibody
TNF alpha (Human) antibody
Overview
| Antigen: | TNF alpha |
| Antibody Type: | Monoclonal |
| Application: | ELISA, Immunoprecipitation (IP) |
| Reactivity: | Human |
| Host: | Mouse |
| Quantity: | |
| Price: | 295,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN138140 |
| Availability: | 7 to 10 days until shipment |
Additional Information: TNF alpha (Human) antibody
| Antigen | TNF alpha |
| Immunogen | In vitro cell culture, protein G purified (Human). |
| Reactivity | Human |
| Antibody Type | Monoclonal |
| Format | Liquid |
| Isotype | IgG2a »Matching secondary antibodies |
| Description | Mouse monoclonal [AS3] to TNF alpha |
| Clone | AS3 |
| Host | Mouse |
| Specificity | Recognizes human TNF alpha. Does not recognize human TNF beta, mouse TNF alpha or a panel of other human cytokines. |
Application Details: TNF alpha (Human) antibody
| Application | ELISA, Immunoprecipitation (IP) |
| Application Notes | ELISA: Use at an assay dependent dilution. This antibody has been used as a coating antibody at 5ug/ml in a two site ELISA with biotin conjugated TNF alpha antibody. Typically the ELISA has a working range of 0-10000 pg/ml and a sensitivity of 5 pg/ml with the appropriate standard. Recommended sample volumes are 25-200 ul. Recovery of human TNF alpha in human serum is typically >85% in the ELISA. IP: Use at an assay dependent dilution. This antibody has been used to immunoprecipitate >90% TNF alpha in solution using a double-antibody method (Midgley AR & Hepburn MR Use of the double-antibody method to separate antibody bound from free ligand in radioimmunoassay. Methods Enzymol 70:266-74 (1980). PubMed: 7421592). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Concentration | 1 mg/ml |
| Purity | >95% by SDS-PAGE |
| Buffer | Preservative: 0.08% Sodium Azide. Constituents: PBS |
| Storage | Store at 4C. Aliquot and store at -20C long-term. |
| Restrictions | For research use only |
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