SFPQ antibody

Overview

Antigen:	SFPQ
Antibody Type:	Monoclonal
Host:	Mouse
Quantity:	50 µl (2 mg/ml)
Price:	247,00 € (Plus shipping costs and VAT)
Order number:	ABIN137584
Availability:	7 to 10 days until shipment

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Additional Information: SFPQ antibody

Antigen	SFPQ
Immunogen	Tissue / cell preparation (lysate of mouse bone marrow-derived stromal cell line.).
Antibody Type	Monoclonal
Format	Liquid
Isotype	IgG1 »Matching secondary antibodies
Description	Essential pre-mRNA splicing factor required early in spliceosome formation. Binds to the mammalian polypyrimidine tracts. Forms a complex with the polypyrimidine tract-binding protein (PTB). Seems to also bind DNA. It is a heterotetramer of two 52 kDa and two 100 kDa subunits. The RNAs that direct protein synthesis in animals and plant cells are synthesized in the nucleus as large precursors (pre-mRNAs). The protein coding sequences in pre-mRNA molecules are arranged in discontinuous segments-exons interspersed with noncoding sequences-introns. In a process termed splicing, these introns are efficiently removed before the pre-mRNA is transported from the nucleus to the cytoplasm, where it is translated into protein. Studies have shown that nuclear pre-mRNA splicing takes place in a multi-component structure termed a spliceosome. The polypyrimidine tract-binding (PTB) protein-associated splicing factor (PSF), which plays an essential role in mammalian spliceosomes, is a ubiquitous n
Clone	B92
Host	Mouse

Application Details: SFPQ antibody

Application Notes	For AP: Use at an assay dependent dilution (see Shav-Tal et al reference). For ICC: Use at an assay dependent dilution (fix cells 2 min in 3% paraformaldehyde with 0.5% Triton X-100, and then 20 min in 3% paraformaldehyde) (see Lee et al reference). For IHC-Fr: Use at an assay dependent dilution (4% paraformaldehyde-0.5% Triton X-100 treated tissue). For IP: Use at an assay dependent dilution (see Lee et al reference). For RIA: Use at an assay dependent dilution (see Lee et al reference). WB: Use at a concentration of 2-4 ug/ml. Detects a band of approximately 47, 49, 100, 68, 120, 100, 80 and 68 kDa (predicted molecular weight: 76 kDa). (See Lee et al reference, Shav-Tal et al reference). Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Concentration	2 mg/ml
Purity	Protein A purified
Buffer	Phosphate-buffered saline, pH 7.4, containing 15 mM sodium azide
Restrictions	For research use only

Publications: SFPQ antibody

Publications

Shav-Tal, Lee, Bar-Haim et al.:
"Enhanced proteolysis of pre-mRNA splicing factors in myeloid cells."
In: Experimental hematology , Vol. 28, Issue 9, pp. 1029-38, 2000/01 (PubMed)

Lee, Shav-Tal, Peled et al.:
"A hematopoietic organ-specific 49-kD nuclear antigen: predominance in immature normal and tumor granulocytes and detection in hematopoietic precursor cells."
In: Blood , Vol. 87, Issue 6, pp. 2283-91, 1996/01 (PubMed)

External Information: SFPQ antibody

Google Scholar	Find more references for clone B92 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen SFPQ (Bioinformatic Harvester (EMBL))