Caspase 4 (Human) antibody

Overview

Antigen	Caspase 4
Immunogen	Synthetic peptide: KERAEEIYPIKERNN conjugated to KLH, corresponding to amino acids 119-133 of Human Caspase 4.
Reactivity	Human
Antibody Type	Monoclonal
Format	Liquid
Isotype	IgG1 »Matching secondary antibodies
Description	Caspase 4 is a 377 amino acids protein (43 kDa) that belongs to the Ced3/ICE family of cystein proteases. Caspase 4 is an upstream caspase with a long prodomain. It undergoes a cleavage upon activation, leading to p20 and p10 subunits which have 67% homology to mature caspase 1 over the equivalent coding sequences. Caspase 4 is capable of cleaving itself and the p30 caspase 1 precursor, and of inducing apoptosis in transfected insect or COS cells. In high concentrations, it can also cleave the nuclear DNA enzyme PARP but the biological relevance of this cleavage is unclear. Caspase 4 mRNA is found in most tissues examined with the exception of the brain. Highest expression is found in spleen and lung, with moderate expression in heart and liver. Low expression is observed with skeletal muscle, kidney, and testis.
Clone	CAS4
Host	Mouse

Application	ELISA, Western Blotting (WB)
Application Notes	ELISA: Use at an assay dependent dilution. WB: Use at a concentration of 1-2 ug/ml. Detects a band of approximately 43 kDa with additional bands at 50 and 20 KDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Concentration	2 mg/ml
Purity	Protein A purified
Buffer	Preservative: 15mM Sodium AzideConstituents: 0.01M PBS. pH 7.4
Restrictions	For research use only

Google Scholar	Find more references for clone CAS4 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Caspase 4 (Bioinformatic Harvester (EMBL))