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Product details for Rhodopsin Kinase 1a/b (Human) antibody
Rhodopsin Kinase 1a/b (Human) antibody
Overview
| Antigen: | Rhodopsin Kinase 1a/b |
| Antibody Type: | Monoclonal |
| Application: | Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB) |
| Reactivity: | Human, Bovine |
| Host: | Mouse |
| Quantity: | |
| Price: | 388,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN146064 |
| Availability: | 7 to 10 days until shipment |
Additional Information: Rhodopsin Kinase 1a/b (Human) antibody
| Antigen | Rhodopsin Kinase 1a/b |
| Reactivity | Human, Bovine |
| Antibody Type | Monoclonal |
| Format | Liquid |
| Isotype | IgG »Matching secondary antibodies |
| Description | The sensation of sight is the result of a cascade of events starting with the interaction of photoactivated rhodopsin with a protein called transducin. Rhodopsin kinase is a G-protein-coupled Ser/Thr kinase, also known as GRK1, which is a key element in the regulation of this cascade. Following phosphorylation by GRK1, arrestin is recruited to phospho-rhodopsin quenching its phototransductive activity by preventing further interaction with transducin. By breaking the cycle of phototransduction, GRK1 plays an important role in the restoration of the system for subsequent visual events. |
| Clone | D11 |
| Host | Mouse |
| Specificity | Detects G-protein-associated rhodopsin kinase 1a/b (GRK1a/b) from human and bovine tissues. This antibody does not cross-react with other G protein-coupled kinases. |
Application Details: Rhodopsin Kinase 1a/b (Human) antibody
| Application | Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB) |
| Application Notes | Immunofluorescence 1:1000 Western Blot 1:1000 By Western blot, this antibody detects an ~60 kDa protein representing GRK 1a/b. GTX22766 detects both in vitro expressed splice variants by Western blot but was not able to detect significant amounts of GRK1b from human retina. Immunofluorescence staining of GRK1a/b in bovine retina with GTX22766 results in intense staining primarily in cone outer segments, but also in rod outer segments. Weak staining is observed in somata and synaptic terminals of cones and inner segments of rods. |
| Concentration | 1 mg/ml |
| Purity | IgG fraction |
| Buffer | Preservative: 0.05% Sodium Azide |
| Storage | Store at 4 C. Aliquot and store at -20 C long-term. |
| Restrictions | For research use only |
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