Cdk4 (Human) antibody

Overview

Antigen:	Cdk4
Antibody Type:	Monoclonal
Application:	Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
Reactivity:	Human, Mouse (Murine), Rat
Host:	Mouse
Quantity:	100 µl
Price:	247,00 € (Plus shipping costs and VAT)
Order number:	ABIN145970
Availability:	7 to 10 days until shipment

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Additional Information: Cdk4 (Human) antibody

Antigen	Cdk4
Immunogen	Recombinant full length protein (Human).
Reactivity	Human, Mouse (Murine), Rat
Antibody Type	Monoclonal
Isotype	IgG2a »Matching secondary antibodies
Description	Regulation of cell cycle progression in eukaryotic cells depends on the expression of cyclins. These proteins form complexes with several different cyclin dependant kinases (CDKs). Within complexes, the cyclin subunit serves a regulatory role, whereas the CDKs have a catalytic protein kinase activity. Complexes of cyclins and CDKs play a key role in cell cycle control. The eukaryotic cell cycle is regulated by the sequential activation of CDKs.
Clone	DCS-31
Host	Mouse
Specificity	Reacts specifically with Cdk4. Does not recognise other Cdk types.

Application Details: Cdk4 (Human) antibody

Application	Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
Application Notes	ICC: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. (recognises a 33kD band, and it may pick up additional weaker bands, using a cultured human tumor cell line extract)Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Purity	Ascites
Buffer	Ascitic fluid with 15mM sodium azide
Storage	Store at 4 C. Aliquot and store at -20 C long-term. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is not recommended.
Restrictions	For research use only

Publications: Cdk4 (Human) antibody

Publications

Matsushime, Ewen, Strom et al.:
"Identification and properties of an atypical catalytic subunit (p34PSK-J3/cdk4) for mammalian D type G1 cyclins."
In: Cell , Vol. 71, Issue 2, pp. 323-34, 1992/01 (PubMed)

Pines, Hunter:
"Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport."
In: The Journal of cell biology , Vol. 115, Issue 1, pp. 1-17, 1991/01 (PubMed)

Freeman, Donoghue:
"Protein kinases and protooncogenes: biochemical regulators of the eukaryotic cell cycle."
In: Biochemistry , Vol. 30, Issue 9, pp. 2293-302, 1991/01 (PubMed)

Lukas, Aagaard, Strauss et al.:
"Oncogenic aberrations of p16INK4/CDKN2 and cyclin D1 cooperate to deregulate G1 control."
In: Cancer research , Vol. 55, Issue 21, pp. 4818-23, 1995/01 (PubMed)

Aagaard, Lukas, Bartkova et al.:
"Aberrations of p16Ink4 and retinoblastoma tumour-suppressor genes occur in distinct sub-sets of human cancer cell lines."
In: International journal of cancer. Journal international du cancer , Vol. 61, Issue 1, pp. 115-20, 1995/01 (PubMed)

Lukas, Parry, Aagaard et al.:
"Retinoblastoma-protein-dependent cell-cycle inhibition by the tumour suppressor p16."
In: Nature , Vol. 375, Issue 6531, pp. 503-6, 1995/01 (PubMed)

Xiong, Zhang, Beach:
"Subunit rearrangement of the cyclin-dependent kinases is associated with cellular transformation."
In: Genes & development , Vol. 7, Issue 8, pp. 1572-83, 1993/01 (PubMed)

Motokura, Arnold:
"Cyclins and oncogenesis."
In: Biochimica et biophysica acta , Vol. 1155, Issue 1, pp. 63-78, 1993/01 (PubMed)

Sherr:
"Mammalian G1 cyclins."
In: Cell , Vol. 73, Issue 6, pp. 1059-65, 1993/01 (PubMed)

Elledge:
"Cell cycle checkpoints: preventing an identity crisis."
In: Science (New York, N.Y.) , Vol. 274, Issue 5293, pp. 1664-72, 1997/01 (PubMed)

External Information: Cdk4 (Human) antibody

Google Scholar	Find more references for clone DCS-31 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Cdk4 (Bioinformatic Harvester (EMBL))