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Product details for Defensin alpha 1-3 (Human) antibody
Defensin alpha 1-3 (Human) antibody
Overview
| Antigen: | Defensin alpha 1-3 |
| Antibody Type: | Monoclonal |
| Application: | Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) |
| Reactivity: | Human |
| Host: | Mouse |
| Quantity: | |
| Price: | 430,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN118975 |
| Availability: | Ships in 3 to 10 days |
Additional Information: Defensin alpha 1-3 (Human) antibody
| Antigen | Defensin alpha 1-3 |
| Reactivity | Human |
| Antibody Type | Monoclonal |
| Format | Purified |
| Isotype | IgG1 »Matching secondary antibodies |
| Clone | DEF3 |
| Host | Mouse |
| Specificity | Defensin 1-3 (HNP-1 to HNP-3) in human neutrophils. It is not known whether it reacts with Defensin-4. A side reaction to elastin has been observed in humans. Species crossreactivity is not known. Defensins have been described in rabbits (NP-1 to NP-5, NP3a = Corticostatin), and in mice (cryptodin). DEF-3 recognises a family of cyclic peptides in neutrophils. Four of these peptides are described in humans (HNP-1 to 4), six in rabbits (NP-1 to 5). Synonyms are MCP-1 for NP-1, MCP-2 for NP-2 and corticostatin for NP-3. The function of these peptides apart from their bactericidal, antifungal, and monocyte chemotactic functions is the inhibition of ACTH - induced corticosteroid synthesis. DEF-3 is an important marker for inflammation typing, and for staining mature neutrophils in immunohistochemistry. Defensins are a group of cyclic peptides containing 29-35 amino acids (MW < 3500) which tend to form aggregates. The molecules are protease resistant. The Defensin content of azurophilic granules in neutrophils is approximately 30% of the total protein. Various functions have been described for Defensins. They are antibacterial, antifungal, chemotactic for monocytes, inhibitory for ACTH-induced corticosteroid synthesis and cytotoxic for cells. Defensins are inhibited by glucosaminoglycans (self protection for cells) and high concentrations of Ca2+ ions. |
Application Details: Defensin alpha 1-3 (Human) antibody
| Application | Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) |
| Application Notes | Flow cytometry with permeabilized cells (use 5 ul of neat antibody to label 106 cells). Immunohistochemistry on frozen (0.2 ug/ml or 1:5000) Fixation: Acetone and formalin fixed paraffin embedded sections (requires pretreatment by enzyme digestion). ELISA (protocol see below) Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Concentration | 1.0 mg/ml |
| Buffer | This antibody is supplied as liquid, purified immunoglobulin fraction in PBS buffer pH 7.2 with 0.09% sodium azide as preservative. |
| Storage | Store short term (up to 2 weeks) at 4°C or aliquot and store at -20°C to -70°C, do not freeze working dilutions. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Research Area | Immunology |
| Restrictions | Research only |
Publications: Defensin alpha 1-3 (Human) antibody
| Publications |
Schluesener, Meyermann: "Neutrophilic defensins penetrate the blood-brain barrier." In: Journal of neuroscience research , Vol. 42, Issue 5, pp. 718-23, 1996/01 (PubMed) |
External Information: Defensin alpha 1-3 (Human) antibody
| Google Scholar | Find more references for clone DEF3 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Defensin alpha 1-3 (Bioinformatic Harvester (EMBL)) |
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