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Product details for DAP Kinase 1 (phospho S308) (Human) antibody
DAP Kinase 1 (phospho S308) (Human) antibody
Overview
| Antigen: | DAP Kinase 1 (phospho S308) |
| Antibody Type: | Monoclonal |
| Application: | Dot Blot (Dot), ELISA, Immunoprecipitation (IP) |
| Reactivity: | Human |
| Host: | Mouse |
| Quantity: | |
| Price: | 271,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN146423 |
| Availability: | 7 to 10 days until shipment |
Additional Information: DAP Kinase 1 (phospho S308) (Human) antibody
| Antigen | DAP Kinase 1 (phospho S308) |
| Immunogen | Synthetic peptide, corresponding to amino acids 303-312 (pSER308) of Human DAP Kinase 1. |
| Reactivity | Human |
| Antibody Type | Monoclonal |
| Format | Liquid |
| Isotype | IgG1 »Matching secondary antibodies |
| Description | Various apoptotic signals use Death Associated Protein Kinase (DAPK) as a downstream effector in different cell types. DAPK is a positive mediator of apoptosis and is widely expressed in many tissues of embryonic and adult origin. DAPK is a Ca2+/calmodulin dependent Ser/Thr kinase that associates with microfilaments. The protein is composed of a multidomain structure. It has a subdomain typical of serine/threonine kinases, a Ca2+/calmodulin regulatory domain, eight ankyrin repeats followed by two P-loop motifs and a typical death domain module. It contains two auto-inhibitory domains one of them Ca2+/calmodulin dependent. In the absence of this latter domain, DAPK is constitutively active. DAPK activity is also regulated by phosphorylation. DAPK was found to be negatively regulated by autophosphorylation at Ser308 which is in the calmodulin regulatory domain. This autophosphorylation, which occurs in cells at the basal state, lowers the affinity of DAPK for calmodulin and thus the k |
| Clone | DKPS308 |
| Host | Mouse |
Application Details: DAP Kinase 1 (phospho S308) (Human) antibody
| Application | Dot Blot (Dot), ELISA, Immunoprecipitation (IP) |
| Application Notes | Dot: Use at a concentration of 1-2 ug/ml, Determined by immunoblotting using 293T (human embryonal kidney) cells transfected with DAPKinase expression vector. ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Concentration | 2 mg/ml |
| Purity | Immunogen affinity purified |
| Buffer | Preservative: 15mM Sodium AzideConstituents: 0.01mM PBS. pH 7.4 |
| Restrictions | For research use only |
External Information: DAP Kinase 1 (phospho S308) (Human) antibody
| Google Scholar | Find more references for clone DKPS308 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen DAP Kinase 1 (phospho S308) (Bioinformatic Harvester (EMBL)) |
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