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Product details for ST2 (FB9) (Human) antibody
ST2 (FB9) (Human) antibody
Overview
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| Antigen: | ST2 (FB9) |
| Antibody Type: | Monoclonal |
| Application: | Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP) |
| Reactivity: | Human |
| Host: | Mouse |
| Quantity: | |
| Price: | 251,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN130564 |
| Availability: | 7 to 10 days until shipment |
Additional Information: ST2 (FB9) (Human) antibody
| Antigen | ST2 (FB9) |
| Immunogen | This antibody was purified from mouse ascites fluid using Protein-A Sepharose. This hybridoma was established by fusion of mouse myeloma cell PAI with Balb/c splenocyte immunized with secreted form of ST2 protein which were purified from culture supernatant of COS7 transfectant cells. |
| Reactivity | Human |
| Antibody Type | Monoclonal |
| Format | Liquid |
| Isotype | IgG1 »Matching secondary antibodies |
| Description | The ST2 gene, also known as T1, Fit1, or DER4, was originally identified as a responsive gene that was highly induced by stimulation of various proliferation-inducing agents including serum, PDGF (platelet-derived growth factor), FGF (fibroblast growth factor), or lysophosphatidic acid in murine fibroblasts (1, 2). Three distinct forms of gene products have been reported and named ST2, ST2V, and ST2V. ST2 is a soluble secreted form of 37.1kD protein which lacks intracellular domain, whereas ST2L is a transmembrane form of 61.5kD protein (the glycosylated forms of ST2 and ST2L are about 57kD and 80kD, respectively). This ST2L protein is very similar to IL-1R (interleukin-1 receptor) type I and II in structure, thus it is considered as a member of the IL-1R family (3, 4). ST2V, which is another novel variant form of human ST2, has been identified recently (5). ST2 proteins are expressed in the wide variety types of human cells, including hematopoietic cells in various stages of differentiation, a population of the peripheral blood mononuclear cells from healthy individuals, glioblastoma and astrocytoma cell lines, and colon cancer cells in addition to fibroblast cell lines (4). Thus ST2 proteins are considered to have some roles in regulating cell growth or proliferation. On the other hand, either definitive functions of ST2 proteins or their ligand molecule(s) which binds to ST2 proteins have remained unclear, though it has been reported that none of IL- 1a, ß , RA (receptor antagonist) binds to ST2 proteins in spite of their structural similarity to IL-1R (3, 4). This indicates that ST2L protein is functionally independent from IL-1R. Furthermore, several studies have shown that ST2L is expressed on the cell surface of Th2 cells but not on the Th1 cells (4, 6), indicating the possibility that ST2L protein participates not only in the regulation of cell growth or proliferation, but also in the immune system including differentiation of T cells or immunological response via helper T cells. From these observations, ST2 proteins are considered to be one of the important proteins participate in various physiological phenomenon, thus further analysis are required to understand its physiological functions. MONOCLONAL Antibody Anti-Human ST2 Code No. Clone Subclass Quantity Form D066-3 FB9 Mouse IgG1 100 µ g Solution in 50% glycerol/PBS |
| Clone | FB9 |
| Host | Mouse |
| Specificity | This antibody detects human ST2 protein on Flowcytometric analysis. |
Application Details: ST2 (FB9) (Human) antibody
| Application | Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP) |
| Application Notes | Preparation for Flowcytometric analysis We use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Prepare the indicated solutions as below. Wash solution: 2% FCS/0.1%NaN 3 -PBS 1st antibody solution: 10 µ g/ml of anti-ST2 antibody in Wash solution. 2nd antibody solution: Anti-Mouse IgG conjugated with FITC(IMMUNOTECH: Cat No. Flow Cytometry:Fluorescence intensity T98G expressing Human ST2L Anti-ST2 ( Flow cytometry: 10 µ g/ml RESEARCH Applications. It is reported that this antibody can also be used on Westernblotting and Immunoprecipitation (Kuroiwa et al., 2000). |
| Buffer | 100 µ g IgG in 100 µ l volume of PBS containing 50% glycerol. No preservative is contained. |
| Restrictions | For Research Use Only |
Publications: ST2 (FB9) (Human) antibody
| Publications |
Tominaga: "A putative protein of a growth specific cDNA from BALB/c-3T3 cells is highly similar to the extracellular portion of mouse interleukin 1 receptor." In: FEBS letters , Vol. 258, Issue 2, pp. 301-4, 1990/01 (PubMed) Lanahan, Williams, Sanders et al.: "Growth factor-induced delayed early response genes." In: Molecular and cellular biology , Vol. 12, Issue 9, pp. 3919-29, 1992/01 (PubMed) |
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