HMGB1/2 (HMG1/2) (Human) antibody

Overview

Antigen:	HMGB1/2 (HMG1/2)
Antibody Type:	Monoclonal
Application:	Western Blotting (WB)
Reactivity:	Human, Mouse (Murine), Rat, Bovine
Host:	Mouse
Quantity:	100 µg
Price:	251,00 € (Plus shipping costs and VAT)
Order number:	ABIN130604
Availability:	7 to 10 days until shipment

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Additional Information: HMGB1/2 (HMG1/2) (Human) antibody

Antigen	HMGB1/2 (HMG1/2)
Immunogen	This antibody was purified from ascites fluid using protein-A Sepharose. The hybridoma of the antibody was established by fusion of mouse myeloma cell PAI with Balb/c mouse lymph node immunized with porcine HMG1.
Reactivity	Human, Mouse (Murine), Rat, Bovine
Antibody Type	Monoclonal
Format	Liquid
Isotype	IgG1 »Matching secondary antibodies
Clone	FBH7
Host	Mouse
Specificity	This antibody reacts with the ~29kDa HMGB1 and ~28kDa HMGB2 by Western blotting.

Application Details: HMGB1/2 (HMG1/2) (Human) antibody

Application	Western Blotting (WB)
Application Notes	SDS PAGE & Western Blotting 1) Suspend 1x10 7 of cells with 1 ml of Laemmli sample buffer and sonicate the sample briefly on ice. Make sure the cells are completely lysed. 2) Boil the samples for 2 minutes and centrifuge. Load 10 µ l of a supernatant (cell lysate) per lane in a 1 mm thick 12.5% SDS-polyacrylamide gel for electrophoresis. 3) Blot the protein to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm 2 for 1 hour in a semi-dry transfer system. (Transfer Buffer - 25 mM Tris, 190 mM glycine, 20% MeOH). See the manufacture's manual for specific transfer procedure. 4) To reduce nonspecific binding, soak the membrane in Block Ace TM buffer for 1 hour at 37°C, or overnight at 4°C. 5) Prepare 1 µ g /ml of anti-HMGB1/2 antibody diluted with 10% Block Ace TM -PBS. Incubate the membrane with the solution for 1 hour at 25°C. (The optimal antibody concentra- ttion will depend on the experimental conditions.) 6) Wash the membrane with 0.2% Tween 20-PBS. (10 minutes x 3 times) 7) Prepare POD-conjugated anti-mouse IgG (MBL, Cat. No. 330) diluted 1:10000 with 10% Block Ace TM -PBS solution as a secondary antibody. Incubate the membrane with the secondary antibody for 1 hour at 25°C. 8) Wash the membrane with 0.2% Tween 20-PBS. (10 minutes x 3 times) 9) Wipe excess buffer from the membrane, then incubate it with appropriate chemiluminescence reagents for 1 minute. Remove extra reagent from the membrane by dabbing with a paper towel, and seal it in plastic wrap. 10) Expose toX-ray film in a dark room for 30 seconds. Develop the film as usual. The conditions for exposure and development may vary. Western blotting: 1 µ g/ml Research Applications.
Buffer	100 micrograms IgG in 100 microliter volume of PBS containing 50% glycerol. No preservative is contained.
Restrictions	For Research Use Only

Publications: HMGB1/2 (HMG1/2) (Human) antibody

Publications

Sobajima, Ozaki, Osakada et al.:
"Novel autoantigens of perinuclear anti-neutrophil cytoplasmic antibodies (P-ANCA) in ulcerative colitis: non-histone chromosomal proteins, HMG1 and HMG2."
In: Clinical and experimental immunology , Vol. 107, Issue 1, pp. 135-40, 1997/01 (PubMed)

Taguchi, Blood, del Toro et al.:
"Blockade of RAGE-amphoterin signalling suppresses tumour growth and metastases."
In: Nature , Vol. 405, Issue 6784, pp. 354-60, 2000/01 (PubMed)

Wang, Bloom, Zhang et al.:
"HMG-1 as a late mediator of endotoxin lethality in mice."
In: Science (New York, N.Y.) , Vol. 285, Issue 5425, pp. 248-51, 1999/01 (PubMed)

Yoshida:
"Mg2+-, Ca2+-dependent unwinding of DNA by poly-L-glutamic acid."
In: Biochemical and biophysical research communications , Vol. 116, Issue 1, pp. 217-21, 1983/01 (PubMed)

External Information: HMGB1/2 (HMG1/2) (Human) antibody

Google Scholar	Find more references for clone FBH7 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen HMGB1/2 (HMG1/2) (Bioinformatic Harvester (EMBL))