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Product details for Fas Ligand (Mouse) antibody
Fas Ligand (Mouse) antibody
Overview
| Antigen: | Fas Ligand |
| Antibody Type: | Monoclonal |
| Application: | Flow Cytometry (FACS) |
| Reactivity: | Mouse (Murine), Human |
| Host: | Hamster |
| Quantity: | |
| Price: | 286,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN130549 |
| Availability: | 7 to 10 days until shipment |
Additional Information: Fas Ligand (Mouse) antibody
| Antigen | Fas Ligand |
| Immunogen | This antibody was purified from mouse ascites fluid using protein-A Sepharose. The hybridoma of the antibody was established by fusion of mouse myeloma cell P3U1with lymphnode cell of an Armenian hamster immunized with W40LFL cells (described below). |
| Reactivity | Mouse (Murine), Human |
| Antibody Type | Monoclonal |
| Format | Liquid |
| Isotype | IgG »Matching secondary antibodies |
| Description | Fas ligand (FasL) is a type II- membrane protein, whose N terminus is in the cytoplasm and its C-terminal region extends into the extracellular space. Its receptor Fas, also termed Apo-1 or CD95, is a cell-surface type I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family. It is known that engagement of Fas by FasL results in apoptotic cell death. Binding of a trimeric FasL to Fas induces trimerization of Fas, and FADD/MORT1 binds to the trimerized Fas cytoplasmic region through the interaction of the respective death domains. Caspase-8 is then recruited to FADD/MORT1 through binding of each death effector domains (DED), which in turn may induce self-activation of the protease domain and eventually leads cell to apoptosis. FasL is predominantly expressed in activated T lymphocytes and Natural Killer (NK) cells, although it is expressed in the tissues of the immune-privilege sites such as the testis and eye. Furthermore, a variety of cancers such as melanomas were found to express FasL in addition to Fas. On the other hand, Fas is expressed in various tissues with abundant expression in the thymus, liver, heart, and kidney. It is reported that Fas/FasL system is involved in the deletion of activated or autoreactive T and B cells. Mature T cells of normal mice are known to die, after they accomplish their tasks. On the contrary, mature T cells from lpr and gld mice, which are deficient of Fas and FasL respectively, do not die after activation, and activated cells accumulate in the lymph nodes and spleens of these mice. Moreover T cell hybridomas activated in the presence of Fas-neutralizing molecule, do not die. In addition to T cells, the Fas-deficient mice accumulate B cells and have elevated levels of immunoglobulins (Ig) of various classes that include anti-ssDNA and anti-dsDNA antibodies. FasL can be used as an immune-suppressive agent. Rejection of transplants is mediated by activated T cells that may express functional Fas. If a transplanted tissue is engineered to express FasL or is co-transplanted with FasL- expressing cells, the transplant may be tolerated. Also Fas/ FasL system has been shown to play a role in human diseases such as fulminant hepatitis, AIDS, cancers and other diseases involving CTL-induced tissue destruction. MONOCLONAL Antibody Anti- Mouse Fas Ligand Code No. Clone Ig type Quantity Form D057-3 FLIM58 Hamster IgG 100 µ g Solution in 50% glycerol/PBS D057-3 January 20, 2000 |
| Clone | FLIM58 |
| Host | Hamster |
| Specificity | This antibody detects mouse FasL of B6, C3H and MRL mouse as well as BALB/c and DBA/1 mouse, but does not detect gld-type mouse FasL mutant by flowcytometic analysis. We have tested the reactivity on flowcytometry using mouse cell line W40LFL which is overexpressor of mouse FasL extracellular region (See ref. 4 for more information about this cell line). Ref. 4 showed neutralizing activity of this antibody to soluble and membrane-bound mouse FasL, however we do not test the neutralizing activity as quality control. Human FasL Mouse FasL control Other species cell 1A12* W40LFL** WR19L*** NT reactivity on Flowcytometry ± + - +: react, ± |
Application Details: Fas Ligand (Mouse) antibody
| Application | Flow Cytometry (FACS) |
| Application Notes | Preparation for Flowcytometric analysis We use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Prepare Wash solution (2% FCS in PBS) and antibody solutions. 1st antibody solution: 5 µ g/mL of anti-FasL antibody in Wash solution. 2nd antibody solution: Anti-Hamster IgG (rabbit, Cat. No. 503-03) diluted x 400 with Wash solution. 3rd antibody solution: fluorescein conjugated anti-Rabbit IgG (Immunotech, Cat. No. IM-0833) diluted x50 with Wash solution. Store this solution avoiding the light at 4 o C until use. 2) Dispense cell suspension at 3 X 10 5 cells/tube. Centrifuge at 3000 rpm for 1 minute at 4 o C in a microcentrifuge. Remove supernatant by careful aspiration. 3) Add 10 µ L of the1st antibody solution. Vortex tube gently to suspend the cells and incubate for 20 minutes at 20 o C. 4) Add 1mL of wash solution and centrifuge at 3000 rpm for 1 minute at 4 o C. Remove supernatant by careful aspiration. 5) Add 20 µ L of the 2nd antibody solution. Vortex tube gently and incubate for 20 minutes at 20 o C. 6) Add 1mL of Wash solution and centrifuge at 3000 rpm for 1 minute at 4 o C. Remove supernatant by careful aspiration. 7) Add 30 µ L of the 3rd antibody solution. Vortex tube gently and incubate for 20 minutes at 20 o C. Keep out light by aluminum foil. 8) Add 1mL of Wash solution and centrifuge at 3000 rpm for 1 minute at 4 o C. Remove supernatant by careful aspiration. 9) Add 500 µ l of Wash buffer. Analyze as soon as possible in a flowcytometer. Flowcytometry: 5 µ g/mL Research Applications. |
| Buffer | 100 µ g IgG in 100 µ L volume of PBS containing 50% glycerol. No preservative is contained. |
| Research Area | CD Antigens, Apoptosis/Necrosis, Virology, Cancer |
| Restrictions | For Research Use Only |
External Information: Fas Ligand (Mouse) antibody
| Google Scholar | Find more references for clone FLIM58 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Fas Ligand (Bioinformatic Harvester (EMBL)) |
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