NKG2-A/C/E-FITC (Mouse) antibody

Overview

Antigen:	NKG2-A/C/E
Antibody Type:	Monoclonal
Application:	Flow Cytometry (FACS)
Conjugate:	FITC
Reactivity:	Mouse (Murine)
Host:	Rat
Quantity:	0,5 mg
Price:	363,00 € (Plus shipping costs and VAT)
Order number:	ABIN135337
Availability:	7 to 10 days until shipment

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Additional Information: NKG2-A/C/E-FITC (Mouse) antibody

Antigen	NKG2-A/C/E
Immunogen	CHO cells transfected with murine NKG2 1, 4
Reactivity	Mouse (Murine)
Antibody Type	Monoclonal
Isotype	IgG2ak »Matching secondary antibodies
Description	Mouse NKG2 (Mr ~ 43 kDa) The monoclonal antibody 20d5 reacts with an epitope common to the NKG2A, NKG2C and NKG2E isoforms of the CD94/NKG2 heterodimer, a member of the c-type lectin family of inhibitory receptors. Mouse CD94/NKG2 is a natural killer (NK) cell receptor for the non-classical MHC class I molecule Qa-1b.2, 3 NKG2 is expressed on NK cells, lymphokine-activated killer (LAK) T cells, and some CD8+ memory T cells. Two-color immunofluorescent staining with the 20d5 antibody reveals two distinct NKG2+ phenotypes within the NK1.1+CD3- spleen cell population: NKG2hi (50%) and NKG2low (50%). With the exception of DBA/2J mice, NKG2 is expressed on NK1.1+ cells from all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL).
Clone	G2-A-C-E-FITC
Host	Rat

Application Details: NKG2-A/C/E-FITC (Mouse) antibody

Application	Flow Cytometry (FACS)
Application Notes	Working Dilutions: Flow Cytometry: Purified (1 ug/10^6 cells), Fluorescein (1 ug/10^6 cells), Biotin (1 ug/10^6 cells), R-phycoerythrin (0.2 ug/10^6 cells). Since applications vary, investigators are advised to determine the optimum working dilution of the product that is appropriate for a specific application.
Conjugate	FITC
Storage	The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Restrictions	For Research Use only

Publications: NKG2-A/C/E-FITC (Mouse) antibody

Publications

Vance, Jamieson, Raulet:
"Recognition of the class Ib molecule Qa-1(b) by putative activating receptors CD94/NKG2C and CD94/NKG2E on mouse natural killer cells."
In: The Journal of experimental medicine , Vol. 190, Issue 12, pp. 1801-12, 2000/01 (PubMed)

Vance, Kraft, Altman et al.:
"Mouse CD94/NKG2A is a natural killer cell receptor for the nonclassical major histocompatibility complex (MHC) class I molecule Qa-1(b)."
In: The Journal of experimental medicine , Vol. 188, Issue 10, pp. 1841-8, 1999/01 (PubMed)

External Information: NKG2-A/C/E-FITC (Mouse) antibody

Google Scholar	Find more references for clone G2-A-C-E-FITC on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen NKG2-A/C/E (Bioinformatic Harvester (EMBL))