Product details for  LECT2 (Human) antibody
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LECT2 (Human) antibody

Overview

Antigen: LECT2
Antibody Type: Monoclonal
Application: Immunoprecipitation (IP)
Reactivity: Human
Host: Mouse, Rat
Quantity: 100 µg
Price: 251,00 €   (Plus shipping costs and VAT)
Order number: ABIN130603
Availability: 7 to 10 days until shipment
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Additional Information:  LECT2 (Human) antibody

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Antigen LECT2
Immunogen This antibody was purified from mouse ascites fluid using protein-A Sepharose. This hybridoma was established by fusion of mouse myeloma PAI with BALB/c lymph node cells immunized with recombinant human LECT2 corresponding to full length amino acids purified from culture fluid of stable CHO transfectant cells.
Reactivity Human
Antibody Type Monoclonal
Format Liquid
Isotype IgG2b »Matching secondary antibodies
Description LECT2: leukocyte cell-derived chemotaxin 2, was originally isolated as a 16-kDa secreted protein having a human neutrophil chemotactic activity. It is indicated that LECT2 protein express in the liver specifically and that several human hepatoma cell lines also express this protein. Furthermore, LECT2 was recently found to be identical to chondromodulin-II, a growth stimulator of chondrocyte cells, thus LECT2 is thought to be a multifunctional protein.
Clone G2A5
Host Mouse, Rat
Specificity This antibody react with human LECT2 antigen on Immunoprecipitation.

Application Details:  LECT2 (Human) antibody

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Application Immunoprecipitation (IP)
Application Notes Immunoprecipitation 1) Prepare two tubes with 50 µ l of culture supernatant of CHO tra nsfectant cells which secretes human LECT2. 2) Add 450 µ l of Extraction Buffer (50 mM HEPES (pH 7.3), 250 mM NaCl, 0.2% NP-40, 5 mM EDTA, 10% glycerol, 2 mM benzamidine, 2 µ g/ml leupeptin, 2 µ g/ml pepstatin A) to each tubes. 3) Add 2 µ g of anti-LECT2 monoclonal antibody to one tube. To the other tube, add 2 µ g of normal mouse IgG as the control. Incubate them by gentle mixing for 30 minutes at 4 o C. 4) Add 20 µ l of protein A sepharose (50% suspension) and incubate them by gentle mixing for 1 hour at 4 o C. 5) After brief centrifuging at 12,000 rpm, remove the supernatant. Add High Salt Buffer (Extraction Buffer containing 0.5 M NaCl) and tap the tube. Repeat this step four times. 6) After adding 20 µ l of SDS-PAGE sample buffer (2ME+), boil them for 3 minutes. After brief centrifuging at 10,000 rpm, resolve 10 µl/lane of the sample by 15% SDS-polyacrylamide gel electrophoresis. (See the manufacture's manual for electrophoresis condition). 7) Blot the protein to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm 2 for 1 hour in a semi -dry transfer system. (Transfer Buffer - 25 mM Tris, 190 mM glycine, 20% methanol). See the manufacture's manual for precise transfer procedure. 8) To reduce nonspecific binding, soak the membrane in Block Ace TM (Snow bland) for 1 hour at room temperature, or overnight at 4 o C. 9) Dilute the anti-LECT2 monoclonal antibody to 1 µg/ml with Dilution Buffer (10% Block Ace TM /PBS). Incubate the membrane with primary antibody dilution for 1 hour at room temperature. 10) Wash the membrane with Washing Buffer (0.2% Tween 20 in PBS) for 10 minutes x 3 times. 11) Incubate the membrane with secondary antibody (1:10,000 diluted horseradish peroxidase conjugated anti Mouse IgG (H+L) goat antibody in Dilution Buffer) for 1 hour at room temperature. 12) Wash the membrane for 10 minutes x 3 times with Washing Buffer. 13) Wipe excess buffer on the membrane, then incubate it with appropriate chemiluminescence reagent for 1 minute. Remove extra reagent from the membrane by dabbing with paper towel, and seal it in plastic wrap. 14) Expose to a X-ray film in a dark room for 30 seconds. Develop the film as usual. The condition for exposure and development may vary. Patent Pending Immunoprecipitation: 2 µ g / 500 µ l of reaction solution Research Applications.
Restrictions For Research Use Only

External Information:  LECT2 (Human) antibody

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Google Scholar Find more references for clone G2A5 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen LECT2 (Bioinformatic Harvester (EMBL))