CFTR antibody

Overview


	Antigen:	CFTR
	Antibody Type:	Monoclonal
	Application:	Western Blotting (WB)
	Host:	Mouse
	Quantity:	100 µg (1 mg/mL)
	Price:	216,00 € (Plus shipping costs and VAT)
	Order number:	ABIN131704
	Availability:	7 to 10 days until shipment

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Additional Information: CFTR antibody

Antigen	CFTR
Immunogen	This antibody was purified from hybridoma (clone GA1) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell Ag8-653 with Balb/c mouse lymphocyte immunized with human recombinant CFTR C-terminal fragment (1387a.a.-1480a.a.).
Antibody Type	Monoclonal
Isotype	IgG1 »Matching secondary antibodies
Description	Cystic Fibrosis (CF) is a common lethal genetic disease characterized by chronic bronchopulmonary disease, elevated sweat electrolytes and insufficient pancreatic function. CF is caused by mutations of the CFTR gene, which encodes the cystic fibrosis transmembrane conductance regulator (CFTR). The CFTR protein is a cAMP-activated chloride channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 which results in abnormal chloride transport. CFTR is structurally similar to multi-drug resistance (Mdr1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.
Clone	GA1
Host	Mouse
Specificity	This antibody reacts with CFTR (200kDa) by Western blotting.

Application Details: CFTR antibody

Application	Western Blotting (WB)
Application Notes	Western blotting: 10 µg / mL for chemiluminescence detection system. Immunoprecipitation: Not recommended.
Concentration	1 mg/mL
Buffer	100 µg IgG in 100 µL volume of PBS containing 50% glycerol, pH 7.2. Contains no preservatives.
Restrictions	For Research Use Only

Publications: CFTR antibody

Publications

Cormet-Boyaka, Di, Chang et al.:
"CFTR chloride channels are regulated by a SNAP-23/syntaxin 1A complex."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 99, Issue 19, pp. 12477-82, 2002/01 (PubMed)

Li, Roy, Dandridge et al.:
"Molecular assembly of cystic fibrosis transmembrane conductance regulator in plasma membrane."
In: The Journal of biological chemistry , Vol. 279, Issue 23, pp. 24673-84, 2004/01 (PubMed)

Naren, Cobb, Li et al.:
"A macromolecular complex of beta 2 adrenergic receptor, CFTR, and ezrin/radixin/moesin-binding phosphoprotein 50 is regulated by PKA."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 100, Issue 1, pp. 342-6, 2003/01 (PubMed)

Images: CFTR antibody

Western blot analysis of CFTR expression in ZR75- 1 cells (1) and MCF7 cells (2) using ABIN131704 .

Image for CFTR antibody (1)

External Information: CFTR antibody

Google Scholar	Find more references for clone GA1 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen CFTR (Bioinformatic Harvester (EMBL))