IL-13R (Human) antibody

Overview


	Antigen:	IL-13 receptor
	Antibody Type:	Monoclonal
	Application:	Flow Cytometry (FACS), Cell-ELISA
	Reactivity:	Human
	Host:	Mouse
	Quantity:	100 ug
	Price:	250,00 € (Plus shipping costs and VAT)
	Order number:	ABIN108718
	Availability:	1 to 2 days until shipment

Hints on ordering

Add to basket

Customer Service

Technical Inquiry
Send product details to colleague
Download Datasheet

Additional Information: IL-13R (Human) antibody

Antigen	IL-13 receptor
Immunogen	GM1E7 was generated by genetic immunisation of BALB/c mice with an immunisation vector containing the cDNA of the human IL-13Ralpha1 chain extracellular domain (U62858).
Reactivity	Human
Antibody Type	Monoclonal
Format	Purified
Isotype	IgG1 »Matching secondary antibodies
Description	Interleukin 13 (IL-13) is a T cell derived cytokine involved in the regulation of inflammatory and immune responses. IL-13Ralpha1 together with IL-4Ralpha forms a functional receptor for both IL-4 and IL-13, which is why these two cytokines share many of their biological activities. The receptor is found on human B cells, monocytes and endothelial cells. However, no functional receptor is expressed on T cells, which explains why IL-13, in contrast to IL-4, fails to induce TH2-cell differentiation. Purified GM1E7 was analysed for potential inhibitory effects on IL-13-induced short-term and long-term cellular responses. To analysise potential antagonistic effects of GM1E7 on IL-13-driven cell proliferation, the IL-13-responsive human cell line TF-1 was employed. Cells were treated with IL-13 in the presence of varied concentrations of purified GM1E7. To prove that GM1E7 specifically inhibits the formation of a signalling competent IL-4R alpha/IL-13R alpha1 heterodimer, but does not interfere with the closely related IL-4R alpha/gamma c complex, a parallel experiment was performed with IL-4 as the mitogen. As shown in Fig. 2, antibody GM1E7 blocks IL-13-dependent cell proliferation completely, but only marginally influences IL-4-activity on TF-1 cells. The IC50 for GM1E7 with regard to IL-13-driven proliferation is in the range of 50 nM.
Clone	GM1E7
Host	Mouse
Specificity	GM1E7 recognises IL-13Ralpha1 transiently expressed on the cell surface of transfected BOSC cells (Fig. 1) as well as the native protein on monocytes (Myrtek et al., 2004).

Application Details: IL-13R (Human) antibody

Application	Flow Cytometry (FACS), Cell-ELISA
Application Notes	GM1E7 has been tested for the use in flow cytometry and cell-ELISA. It is expected that the antibody will be suited for all applications where recognition of the native protein is required. A titration should be performed to determine the optimal concent
Storage	Store the antibody at 4°C. Avoid repeated freezing and thawing.
Research Area	Inflammation
Restrictions	For research use only. Not for diagnostic or therapeutic use. Caution hazardous ingredient: Sodium azide, avoid exposure to skin and eyes, ingestion, and contact with heat, acids, and metals or metal ions. Wash exposed skin with soap and water. Flush eye

Publications: IL-13R (Human) antibody

Publications

Myrtek et al:
"Expression of interleukin-13 receptor alpha 1-subunit on peripheral blood eosinophils is regulated by cytokines."
In: Immunology , Vol. 112(4), pp. 597-604, 2007/01

Krause et al.:
"Blockade of interleukin-13-mediated cell activation by a novel inhibitory antibody to human IL-13 receptor alpha1"
In: Mol Immunol , Vol. 33, pp. 1799-1807, 2007/01

Images: IL-13R (Human) antibody

Specificity testing of GM1E7. BOSC cells were transiently transfected with an expression vector encoding either the extracellular domain of IL-13Ralpha1 or an irrelevant protein. Expression of the constructs was tested with an anti-tag monoclonal antibody

Image for IL-13R (Human) antibody (1)

Specific inhibition of IL-13-dependentproliferation of TF-1 cells by GM-1E7. Samplesof TF-1 cells were incubated for 72 h with 10 nMhuman IL-13 (closed circles) or 10 nM human IL-4(open circles) plus the indicated concentrations ofpurified GM-1E7. Refere

Image for IL-13R (Human) antibody (2)

External Information: IL-13R (Human) antibody

Google Scholar	Find more references for clone GM1E7 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen IL-13R (ANTAGONISTIC) (Bioinformatic Harvester (EMBL))