CEACAM8 (Human) antibody

Overview


	Antigen:	CEACAM8
	Antibody Type:	Monoclonal
	Application:	Flow Cytometry (FACS), Cell-ELISA
	Reactivity:	Human
	Host:	Mouse
	Quantity:	100 ug
	Price:	250,00 € (Plus shipping costs and VAT)
	Order number:	ABIN179728
	Availability:	1 to 2 days until shipment

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Additional Information: CEACAM8 (Human) antibody

Antigen	CEACAM8
Immunogen	Genetic immunization with cDNA encoding human CEACAM8
Reactivity	Human
Antibody Type	Monoclonal
Format	Purified
Isotype	IgG1 »Matching secondary antibodies
Description	CEA-related cell adhesion molecule 8 (CEACAM8, CD66b) belongs to the carcinoembryonic antigen (CEA) gene family (1,3,4). It encodes a glycosylphosphatidylinositol (GPI)-linked glycoprotein with a Mr of 95,000 which is expressed in cells of the granulocyte-lineage. It is expressed in neutrophils and eosinophils and is characterized as a granulocyte-specific activation antigen. CEACAM8 could serve as a marker for granulocyte activities (2). Like all members of the CEA family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by two immunoglobulin constant-like A and B do-mains.
Clone	GM2H6
Host	Mouse

Application Details: CEACAM8 (Human) antibody

Application	Flow Cytometry (FACS), Cell-ELISA
Application Notes	FACS: 1.2ug/10^6 cells, Cell-ELISA: 1:200 to 1:400
Purity	Green curves). An irrelevant monoclonal antibody served asa negative control (black curves). For specificity testing, protein G-purified GM-2H6 was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM8 transfected cells (red curve).
Purification	PSG: BAP3
Storage	Short term: 2°C - 8°C. long term: -20°C (avoid repeated freezing and thawing)
Restrictions	For research use only. Not for diagnostic or therapeutic use. Caution hazardous ingredient: Sodium azide, avoid exposure to skin and eyes, ingestion, and contact with heat, acids, and metals or metal ions. Wash exposed skin with soap and water. Flush eye

Images: CEACAM8 (Human) antibody

FACS analysis of BOSC23 cells using GM2H6. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM8 (red curve) or an irrelevant protein (control transfectant). Binding of GM-0512 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM8 transfected cells.

Image for CEACAM8 (Human) antibody (1)

BOSC23 cells were transiently transfected with expression vectorscontaining either the cDNA of CEACAM1, CEACAM3-8 or PSG. The latter expressed as a membrane bound fusion protein. Expression of the constructswas tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1,3,4,5 and 6: D14HD11

Image for CEACAM8 (Human) antibody (2)

CEACAM8:Tet2

Image for CEACAM8 (Human) antibody (3)

External Information: CEACAM8 (Human) antibody

Google Scholar	Find more references for clone GM2H6 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen CEACAM8 (Bioinformatic Harvester (EMBL))