CEACAM1 (Human) antibody

Overview


	Antigen:	CEACAM1 (biliary glycoprotein I, BGP, CD66a)
	Antibody Type:	Monoclonal
	Application:	Flow Cytometry (FACS), Cell-ELISA, Immunohistochemistry (IHC)
	Reactivity:	Human
	Host:	Mouse
	Quantity:	100 ug
	Price:	250,00 € (Plus shipping costs and VAT)
	Order number:	ABIN108725
	Availability:	1 to 2 days until shipment

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Additional Information: CEACAM1 (Human) antibody

Antigen	CEACAM1 (biliary glycoprotein I, BGP, CD66a)
Immunogen	GM8G5 was generated by genetic immunisation of BALB/c mice with an immunisation vector containing the cDNA of the human CEACAM1-A2 domain. The antibody was purified by protein G affinity chromatography from cell culture supernatants and is routinely test
Reactivity	Human
Antibody Type	Monoclonal
Format	Purified
Isotype	IgG1 »Matching secondary antibodies
Description	CEACAM1 (BGP) belongs to the carcinoembryonic antigen (CEA) gene family. It is expressed on cells of epithelial and myeloid origin. CEACAM1 expression is downregulated in many tumors indicating a tumor - suppressive function. The antitumor effect may be due to inhibition of tumor angiogenesis, possibly by increased secretion of antiangiogenic molecules from the cells. Like all members of the CEACAM family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by two immunoglobulin constant-like A (A1, A2) and one B domain. While the N, A1 and B domains can also be found in other CEA-family members, the A2 domain of CEACAM1 differs from those found in other CEACAM.
Clone	GM8G5
Host	Mouse
Specificity	GM8G5 recognises CEACAM1 transiently expressed on the cell surface of transfected BOSC cells (Fig. 1) as well as the naturally expressed protein (Fig. 2, kindly provided by Dr. R. Riesenberg, Labor für Tumorimmunologie, Urologische Klinik, Klinikum Grossh

Application Details: CEACAM1 (Human) antibody

Application	Flow Cytometry (FACS), Cell-ELISA, Immunohistochemistry (IHC)
Application Notes	GM8G5 has been tested for the use in flow cytometry, cell-ELISA and immunohistology on cryo-sections (Fig. 3, kindly provided by Prof. M. Neumaier, Institut für Klinische Chemie, Universitätsklinikum Mannheim). It is expected that the antibody will be su
Storage	Store the antibody at 4°C. Avoid repeated freezing and thawing.
Research Area	Receptors, Immunology, Cancer, Receptors, Cell/Tissue Markers, Cancer
Restrictions	For research use only. Not for diagnostic or therapeutic use. Caution hazardous ingredient: Sodium azide, avoid exposure to skin and eyes, ingestion, and contact with heat, acids, and metals or metal ions. Wash exposed skin with soap and water. Flush eye

Images: CEACAM1 (Human) antibody

Specificity testing of GM8G5. BOSC cells were transiently transfected with expression vectors containing the cDNA of either CEACAM1, 3, 4, 5, 6, 7 or 8. Expression of the constructs was tested with monoclonal antibodies known to recognise the correspondi

Image for CEACAM1 (Human) antibody (1)

GM8G5 on renal cell carcinoma (RCC). Hybridoma supernatant of GM8G5 recognises CEACAM1 expressed on the surface of native RCC (orange curve) and on RCC stimulated with IFN-gamma (red curve). PBS served as negative control (black curve)

Image for CEACAM1 (Human) antibody (2)

Immunohistological staining of colon tissue with GM8G5. Cryosections of colon tissue were stained with 10 ug/ml GM8G5 diluted in PBS containing 2.5% horse serum (B). PBS 2.5% horse serum served as negative control (A). Arrows indicate representative sect

Image for CEACAM1 (Human) antibody (3)

External Information: CEACAM1 (Human) antibody

Google Scholar	Find more references for clone GM8G5 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen CEACAM1 (CD66a) (Bioinformatic Harvester (EMBL))