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Product details for Melanoma (IgG1/k) (Human) antibody
Melanoma (IgG1/k) (Human) antibody
Overview
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| Antigen: | Melanoma (IgG1/k) |
| Antibody Type: | Monoclonal |
| Application: | Immunohistochemistry (IHC) |
| Reactivity: | Human |
| Host: | Mouse |
| Quantity: | |
| Price: | 271,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN146446 |
| Availability: | 7 to 10 days until shipment |
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Additional Information: Melanoma (IgG1/k) (Human) antibody
| Antigen | Melanoma (IgG1/k) |
| Immunogen | HMB45-Pigmented melanoma metastases from lymphnodes.MART-1-Recombinant human MART-1 protein.Tyrosinase-Recombinant tyrosinase protein. |
| Reactivity | Human |
| Antibody Type | Monoclonal |
| Format | Liquid |
| Description | The HMB45 clone reacts with a neuraminidase-sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. The HMB45-reactive antigen is present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells and is thought to be oncofetal in nature. This antibody has been shown to label the majority of melanomas. The MART-1/Melan A recognizes a protein of 18kDa, identified at MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. Melan-A is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions. Studies have also shown that MART-1 is more sensitive than HMB45 when labeling metastatic melanomas. Tyrosinase is a key enzyme involved in the initial stages of melanin biosynthesis. Studies have shown Tyrosinase to be a more sensitive marker when compared to HMB45 and MART-1. It has also shown to label a higher percentage of desmoplastic melanomas than HMB45. The combination of HMB45 and th |
| Clone | HMB45 (IgG1-k)-M2-7C10 (IgG2b)-M2-9E3 (IgG2b)-T311 (IgG2a) |
| Host | Mouse |
Application Details: Melanoma (IgG1/k) (Human) antibody
| Application | Immunohistochemistry (IHC) |
| Application Notes | IHC: For an ABC system, dilute 1/25-1/50 and incubate for 30-60 minutes at RT. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Buffer | Preservative: Sodium AzideConstituents: Tissue culture supernatant |
| Restrictions | For research use only |
External Information: Melanoma (IgG1/k) (Human) antibody
| Google Scholar | Find more references for clone HMB45 (IgG1-k)-M2-7C10 (IgG2b)-M2-9E3 (IgG2b)-T311 (IgG2a) on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Melanoma (IgG1/k) (Bioinformatic Harvester (EMBL)) |
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