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Product details for Melanoma (Human) antibody
Melanoma (Human) antibody
Overview
| Antigen: | Melanoma |
| Antibody Type: | Monoclonal |
| Application: | Immunohistochemistry ((Frozen Sections) (IHC) (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) |
| Reactivity: | Human, Mouse (Murine), Rat |
| Host: | Mouse |
| Quantity: | |
| Price: | 271,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN144124 |
| Availability: | 7 to 10 days until shipment |
Additional Information: Melanoma (Human) antibody
| Antigen | Melanoma |
| Immunogen | HMB45-Pigmented melanoma metastases from LNMART-1-Recombinant human MART-1 protein |
| Reactivity | Human, Mouse (Murine), Rat |
| Antibody Type | Monoclonal |
| Isotype | IgG »Matching secondary antibodies |
| Description | HMB-45 and MART-1 are coexpressed in the majority of melanomas, as well as uniquely expressed in certain cases. Thus, the HMB-45 and MART-1 cocktail is potentially more sensitive than HMB-45 or MART-1 alone. MART-1 is a cocktail of clones DT101 and BC199. The combination of HMB-45 and the MART-1 cocktail make this triple antibody cocktail a first-order pan melanoma screener. |
| Clone | HMB45-DT101-BC199 |
| Host | Mouse |
| Specificity | The HMB-45 clone reacts with a neuraminidase-sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. The HMB45-reactive antigen is present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and me |
Application Details: Melanoma (Human) antibody
| Application | Immunohistochemistry ((Frozen Sections) (IHC) (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) |
| Application Notes | IHC:For an ABC system, dilute 1:50-100 and incubate for 30-60 minutes at RT. For formalin fixed paraffin embedded sections, the staining intensity of this melanoma cocktail is enhanced by performing heat mediated antigen retrieval using a high pH buffer. The HMB-45 portion of this antibody will stain with citrate buffer, pH 6.0, however, data shows the MART-1 portion of this cocktail may be negative unless a high pH buffer is used. We therefore, strongly recommend that you do not use citrate buffer for this antibody cocktail. It is sometimes difficult to interpret DAB stained melanomas due to endogenous pigment, therefore we recommend you substitute an AEC or a Fast Red substrate protocol |
| Purity | N/a |
| Buffer | Preservative: Proprietary, not sodium azide or thimerosal. Constituents: PBS, Tissue Culture Supernatant. pH 7.3, Protein carrier. |
| Storage | Store at 4 C. |
| Research Area | Cancer |
| Restrictions | For research use only |
External Information: Melanoma (Human) antibody
| Google Scholar | Find more references for clone HMB45-DT101-BC199 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Melanoma (Bioinformatic Harvester (EMBL)) |
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