gp130 (IL-6Rb subunit (Mouse) antibody

Overview


	Antigen:	Gp130 (IL-6Rb subunit
	Antibody Type:	Monoclonal
	Application:	Western Blotting (WB), Immunoprecipitation (IP), ELISA, Flow Cytometry (FACS)
	Reactivity:	Mouse (Murine), Human
	Host:	Mouse
	Quantity:	100 µg (1 mg/mL)
	Price:	251,00 € (Plus shipping costs and VAT)
	Order number:	ABIN130506
	Availability:	7 to 10 days until shipment

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Additional Information: gp130 (IL-6Rb subunit (Mouse) antibody

Antigen	Gp130 (IL-6Rb subunit
Immunogen	This antibody was purified from mouse ascites fluid using protein G agarose. This hybridoma (clone HMß1) was established by fusion of a mouse myeloma cell XA653 with Armenian hamster splenocyte immunized with mouse CD130 transfected 293T cells.
Reactivity	Mouse (Murine), Human
Antibody Type	Monoclonal
Format	Lyophilised
Isotype	IgG »Matching secondary antibodies
Description	The interleukin-6 (IL-6) cytokine family, including IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1), and B-cell stimulatory factor 3, are also known as gp130 cytokines because they all share gp130 as a common transducer protein within their functional receptor complexes. In the case of IL-6, binding of the ligand to its specific a-receptor (IL-6Ra/CD126) induces dimerization of gp130, activation of the gp130-associated Janus kinases Jak1, Jak2 and Tyk2 and phosphorylation of gp130 at cytoplasmic tyrosine residues. These phosphotyrosines serve as docking sites for SH2-domain-containing proteins such as STAT1, STAT3, the protein tyrosine phosphatase SHP2 and the suppressor of cytokine signaling 3 (SOCS3). The subsequently tyrosine phosphorylated STAT homo- or heterodimers translocate to the nucleus where they bind to enhancer sequences of IL-6-inducible genes. Both IL-6Ra and gp130 have soluble forms. Although soluble gp130 inhibits the effect of IL-6, the soluble IL-6Ra promotes the effect of IL-6.
Clone	HMb1
Host	Mouse
Specificity	This antibody reacts with mouse CD130 antigen on Western blotting, Immunoprecipitation, ELISA and Flow cytometry. It doesn’t react with mouse CD126 antigen.

Application Details: gp130 (IL-6Rb subunit (Mouse) antibody

Application	Western Blotting (WB), Immunoprecipitation (IP), ELISA, Flow Cytometry (FACS)
Application Notes	Flow cytometric analysis for floating cells We recommend Fisher tubes or equivalents as reaction tubes for all steps described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and 0.1% NaN 3 ]. 2) Resuspend the cells with washing buffer (5x10 6 cells/mL). 3) Add 50 µL of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute at room temperature (20~25 o C). Remove supernatant by careful aspiration. 4) Add 10 µL of normal goat serum containing 1 mg/mL normal human IgG and 0.1% NaN 3 to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature. 5) Add 40 µL of mouse CD130 (HMß1) (25 µg/mL) diluted with the washing buffer. Mix well and incubate for 30 minutes at room temperature. 6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration. 7) Add 30 µL of 1:100 FITC conjugated anti-hamster IgG (BD Pharmingen, code no. 554011) diluted with the washing buffer. Mix well and incubate for 15 minutes at room temperature. 8) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration. 9) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. (Positive control for Flow cytometry, transfectant) 070330-2 Western blotting: Not tested. Immunoprecipitation: Not tested. ELISA: Not tested. It is reported that this monoclonal antibody can be used in these applications in the reference 1) and 2). Immunohistochemistry: Not tested. Immunocytochemistry: Not tested. Flow cytometry: 10 µg/mL (final concentration)
Concentration	1 mg/mL
Buffer	100 µg IgG in 100 µL volume of PBS containing 50% glycerol, pH 7.2. Contains no preservatives.
Restrictions	For Research Use Only

Publications: gp130 (IL-6Rb subunit (Mouse) antibody

Publications

Hirano, Matsuda, Nakajima:
"Signal transduction through gp130 that is shared among the receptors for the interleukin 6 related cytokine subfamily."
In: Stem cells (Dayton, Ohio) , Vol. 12, Issue 3, pp. 262-77, 1994/01 (PubMed)

Matsuda, Yamanaka, Hirano:
"Interleukin-6-induced tyrosine phosphorylation of multiple proteins in murine hematopoietic lineage cells."
In: Biochemical and biophysical research communications , Vol. 200, Issue 2, pp. 821-8, 1994/01 (PubMed)

Matsuda, Hirano:
"Establishment of the ELISA for murine soluble gp130, a signal transducer for the IL-6 family cytokine, and its detection in the ascitic fluids of tumor-bearing mice."
In: Biochemical and biophysical research communications , Vol. 202, Issue 1, pp. 637-42, 1994/01 (PubMed)

Yin, Taga, Tsang et al.:
"Involvement of IL-6 signal transducer gp130 in IL-11-mediated signal transduction."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 151, Issue 5, pp. 2555-61, 1993/01 (PubMed)

Images: gp130 (IL-6Rb subunit (Mouse) antibody

Flow cytometric analysis of mouse CD130 expression on transfectant cells (right) and parental cells (left). Open histogram indicates the reaction of isotypic control to the cells. Shaded histograms indicate the reaction of ABIN130506 to the cells.

Image for gp130 (IL-6Rb subunit (Mouse) antibody (1)

External Information: gp130 (IL-6Rb subunit (Mouse) antibody

Google Scholar	Find more references for clone HMb1 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen gp130 (IL-6Rb subunit (Bioinformatic Harvester (EMBL))