Syntaxin-1 (Human) antibody

Overview


	Antigen:	Syntaxin-1
	Antibody Type:	Monoclonal
	Application:	Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
	Reactivity:	Human, Mouse (Murine), Rat, Bovine, Rabbit
	Host:	Mouse
	Quantity:	100 µg (1 mg/mL)
	Price:	216,00 € (Plus shipping costs and VAT)
	Order number:	ABIN131691
	Availability:	7 to 10 days until shipment

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Additional Information: Syntaxin-1 (Human) antibody

Antigen	Syntaxin-1
Immunogen	This antibody was purified from hybridoma (clone HPC-1) supernatant using protein A agarose beads. This hybridoma was established by fusion of mouse myeloma cell NS1 with Balb/c mouse splenocyte immunized with a synaptosomal plasma membrane fraction from adult rat hippocampus.
Reactivity	Human, Mouse (Murine), Rat, Bovine, Rabbit
Antibody Type	Monoclonal
Isotype	IgG1 »Matching secondary antibodies
Description	Syntaxin 1, also known as HPC-1, is a 35 kDa integral membrane protein that is abundantly expressed in neurons and neuroendocrine cells. Syntaxin is essential for synaptic vesicle fusion and it interacts with several other proteins important for synaptic function, including its partners in the fusion complex (Synaptobrevin/VAMP, SNAP-25, a-SNAP, synaptotagmin, Munc-18/n-Sec1, and Ca2+ - channels). Syntaxin is concentrated in synapses and synaptic vesicles where it is a component of the SNARE complex.
Clone	HPC-1-STX-01
Host	Mouse
Specificity	This antibody reacts with 35 kDa membrane protein syntaxin-1, expressed predominantly in neuronal tissues on Western blotting.

Application Details: Syntaxin-1 (Human) antibody

Application	Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes	Western blotting: 0.1 µg/mL for chemiluminescence detection system. Immunoprecipitation: 2 µg / 200 µL of cell extract. Immunohistochemistry: 5 µg/mL Heat treatment is necessary for paraffin embedded sections. Microwave oven: 2 times for 10 minutes each in citrate buffer (pH 6.5)
Concentration	1 mg/mL
Buffer	100 µg IgG in 100 µL volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Restrictions	For Research Use Only

Publications: Syntaxin-1 (Human) antibody

Publications

Graff, Castrop, Bauer et al.:
"Expression of vesicular monoamine transporters, synaptosomal-associated protein 25 and syntaxin1: a signature of human small cell lung carcinoma."
In: Cancer research , Vol. 61, Issue 5, pp. 2138-44, 2001/01 (PubMed)

Yang, Larsson, Braenstroem et al.:
"Syntaxin 1 interacts with the L(D) subtype of voltage-gated Ca(2+) channels in pancreatic beta cells."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 96, Issue 18, pp. 10164-9, 1999/01 (PubMed)

Foletti, Lin, Finley et al.:
"Phosphorylated syntaxin 1 is localized to discrete domains along a subset of axons."
In: The Journal of neuroscience : the official journal of the Society for Neuroscience , Vol. 20, Issue 12, pp. 4535-44, 2000/01 (PubMed)

Galli, McPherson, De Camilli:
"The V0 sector of the V-ATPase, synaptobrevin, and synaptophysin are associated on synaptic vesicles in a Triton X-100-resistant, freeze-thawing sensitive, complex."
In: The Journal of biological chemistry , Vol. 271, Issue 4, pp. 2193-8, 1996/01 (PubMed)

Images: Syntaxin-1 (Human) antibody

Immunoprecipitation of syntaxin-1 from mouse brain extracts with ABIN131691 (1) or mouse IgG1 (2). After immunoprecipitation with the antibody, the immunocomplex was resolved on SDS-PAGE and immunoblotted with ABIN131691.

Image for Syntaxin-1 (Human) antibody (1)

Western blot analysis of syntaxin-1 expression in mouse brain extracts (1) and PC12 cells (2) using ABIN131691.

Image for Syntaxin-1 (Human) antibody (2)

Immunohistochemical detection of syntaxin-1 on paraffin embedded section of a human spinal cord with ABIN131691.

Image for Syntaxin-1 (Human) antibody (3)

External Information: Syntaxin-1 (Human) antibody

Google Scholar	Find more references for clone HPC-1-STX-01 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Syntaxin-1 (Bioinformatic Harvester (EMBL))