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Product details for Intra Acrosomal Protein (Human) antibody
Intra Acrosomal Protein (Human) antibody
Overview
| Antigen: | Intra Acrosomal Protein |
| Antibody Type: | Monoclonal |
| Application: | Immunocytochemistry (ICC), Western Blotting (WB) |
| Reactivity: | Human |
| Host: | Mouse |
| Quantity: | |
| Price: | 291,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN145545 |
| Availability: | 7 to 10 days until shipment |
Additional Information: Intra Acrosomal Protein (Human) antibody
| Antigen | Intra Acrosomal Protein |
| Immunogen | Tissue / cell preparation (Human). Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. |
| Reactivity | Human |
| Antibody Type | Monoclonal |
| Format | Liquid |
| Isotype | IgM »Matching secondary antibodies |
| Description | This protein is a testis-specific, differentiation antigen, acrosomal vesicle protein 1, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. The gene encoding this protein consists of 4 exons and its alternative splicing generates 11 distinct transcripts, which encode protein isoforms ranging from 81 to 265 amino acids. The longest transcript is the most abundant, comprising 53-72% of the total acrosomal vesicle protein 1 messages, the second largest transcript comprises 15-32%, the third and the fourth largest transcripts account for 3.4-8.3% and 8.7-12.5%, respectively, and the remaining 7 transcripts combined account for < 1% of the total acrosomal vesicle protein 1 message. It is suggested that phenomena of cryptic splicing and exon skipping occur within this gene. The acrosomal vesicle protein 1 may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine |
| Clone | Hs-14 |
| Host | Mouse |
| Specificity | The antibody reacts with human intra-acrosomal protein (220 kDa). |
Application Details: Intra Acrosomal Protein (Human) antibody
| Application | Immunocytochemistry (ICC), Western Blotting (WB) |
| Application Notes | ICC: Use at an assay dependant dilution. Designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought). WB: Use at an assay dependant dilution. Detects a band of approximately 220 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Concentration | 1 mg/ml |
| Purity | >95% by SDS-PAGE |
| Buffer | Preservative: 15mM Sodium AzideConstituents: PBS. pH 7.4 |
| Storage | Store at +4 C for 6 months. Do not freeze. |
| Restrictions | For research use only |
External Information: Intra Acrosomal Protein (Human) antibody
| Google Scholar | Find more references for clone Hs-14 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Intra Acrosomal Protein (Bioinformatic Harvester (EMBL)) |
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