BASKET
HELP
Product details for C5b-9 (Human) antibody
C5b-9 (Human) antibody
Overview
| Antigen: | Complement SC5b-9 (TCC / MAC) |
| Antibody Type: | Monoclonal |
| Application: | Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB) |
| Reactivity: | Human, Monkey, Pig (Porcine) |
| Host: | Mouse |
| Quantity: | |
| Price: | 410,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN153081 |
| Availability: | Ships in 7 to 10 days |
Additional Information: C5b-9 (Human) antibody
| Antigen | Complement SC5b-9 (TCC / MAC) |
| Immunogen | Purified human MAC. Cellular Location: Secreted |
| Reactivity | Human, Monkey, Pig (Porcine) |
| Antibody Type | Monoclonal |
| Format | Supernatant |
| Isotype | IgG2a »Matching secondary antibodies |
| Clone | AE11 |
| Host | Mouse |
| Specificity | Reacts with a neoepitope on poly C9 complement factor. This neoepitope is exposed inthe solid phase and membrane form and in the fluid phase form of the terminalcomplement complex, but not in native C9. The antibody reacts with both membranebound and adsorbed terminal complement complex. In Western blotting ab15984 labelsthe poly C9 and to a lesser degree the C9 but does not recognize the C5, C6, C7 or theC8 components. Cross-reacts with various mammals including Human, Baboon and Pig. It does notreact with rat. Not yet tested in other species. |
Application Details: C5b-9 (Human) antibody
| Application | Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB) |
| Application Notes | Suggested working dilutions *ELISA, immunohistochemistry-Fr, Western Blot * Investigator should determine optimal working dilutions. IHC-Fr: Use at a dilution of 1/25 - 1/50. WB: Use at an assay dependent dilution.Testedin peptide ELISA.Using ab15984 tubuli of normal kidney show a scattered weakstaining, while glomeruli are negative. ab15984 also detects SC5b-9 produced byalveolar macrophages in serum free cultures. It reveals strong granular staining ofdeposits of TCC in kidney biopsies (e.g. glomeruli and tubuli).Not tested in otherapplications.Optimal dilutions/concentrations should be determined by the end user. * Other applications have not been tested. Positive Control:Follicular dendritic cells in germinal centres of secondary lymphoid follicles of reactivelymphoid |
| Buffer | 500 microlitres Preservative: 15mM Sodium Azide15mM Sodium AzideConstituents: 0.05M Tris HCl. pH 7.2 |
| Research Area | Complement Factors |
| Restrictions | For Research Use only |
External Information: C5b-9 (Human) antibody
| Google Scholar | Find more references for clone aE11 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Complement SC5b-9 (TCC / MAC) (Bioinformatic Harvester (EMBL)) |
browse antibodies by
Antigens
Secondary Antibodies
Research areas
Clones
Hosts
Applications
Reactivity
Supplier
Antigens
Secondary Antibodies
Research areas
Clones
Hosts
Applications
Reactivity
Supplier
