H-2Kd/H-2Dd-Biotin (Mouse) antibody

Overview

Antigen:	H-2Kd/H-2Dd
Antibody Type:	Monoclonal
Application:	Flow Cytometry (FACS), Functional Studies-Blocking (FUNC-B)
Conjugate:	Biotin
Reactivity:	Mouse (Murine)
Host:	Mouse
Quantity:	0,5 mg
Price:	289,00 € (Plus shipping costs and VAT)
Order number:	ABIN135418
Availability:	7 to 10 days until shipment

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Additional Information: H-2Kd/H-2Dd-Biotin (Mouse) antibody

Antigen	H-2Kd/H-2Dd
Immunogen	C3HBDF1 mouse splenocytes
Reactivity	Mouse (Murine)
Antibody Type	Monoclonal
Isotype	IgG2a »Matching secondary antibodies
Description	An epitope in the 3 domain that is common to H-2Kd and H-2Dd The classical MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic chains noncovalently associated with the invariant 2-microglobulin.3,4 These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.5 MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.3,6 The monoclonal antibody 34-1-2S binds to a common determinant in the 3 domains of H-2Kd and H-2Dd in the presence or absence of 2 microglobulin.7,8 It cross reacts with the 3 domain of H-2Kb.1,
Clone	D-H-2Dd-BIOT
Host	Mouse

Application Details: H-2Kd/H-2Dd-Biotin (Mouse) antibody

Application	Flow Cytometry (FACS), Functional Studies-Blocking (FUNC-B)
Application Notes	Working Dilutions: Flow Cytometry: Purified (1 ug/10^6 cells), Fluorescein (1 ug/10^6 cells), Biotin (1 ug/10^6 cells), R-phycoerythrin (0.1 ug/10^6 cells). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.
Conjugate	Biotin
Storage	The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Restrictions	For Research Use only

Publications: H-2Kd/H-2Dd-Biotin (Mouse) antibody

Publications

Ozato, Hansen, Sachs:
"Monoclonal antibodies to mouse MHC antigens. II. Antibodies to the H-2Ld antigen, the products of a third polymorphic locus of the mouse major histocompatibility complex."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 125, Issue 6, pp. 2473-7, 1981/01 (PubMed)

External Information: H-2Kd/H-2Dd-Biotin (Mouse) antibody

Google Scholar	Find more references for clone d-H-2Dd-BIOT on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen H-2Kd/H-2Dd (Bioinformatic Harvester (EMBL))