Product details for  HIF-1a (Bovine) antibody
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HIF-1a (Bovine) antibody

Overview

Antigen: Hypoxia-inducible Factor 1 alpha (HIF-1 alpha)
Antibody Type: Monoclonal
Application: Immunohistochemistry (Frozen Sections) (IHC) (fro), Immunohistochemistry (paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunoblotting (IB)
Reactivity: Bovine, Ferret, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat, Sheep (Ovine)
Host: Cell Max
Quantity: 0.1 ml (3 mg/ml)
Price: 300,00 €   (Plus shipping costs and VAT)
Order number: ABIN151053
Availability: Ships in 7 to 10 days
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Additional Information:  HIF-1a (Bovine) antibody

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Antigen Hypoxia-inducible Factor 1 alpha (HIF-1 alpha)
Immunogen Fusion protein containing amino acids 432-528 of human HIF-1alpha Cellular Location: HIF-1 is a nuclear protein that activates gene transcription in response to reducedcellular O2 concentration. Activates transcription of EPO, VEGF, iNOS, hemeoxygenase 1 and other critical intracellular responses to hypoxia.
Reactivity Bovine, Ferret, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat, Sheep (Ovine)
Antibody Type Monoclonal
Format Purified
Isotype IgG2b »Matching secondary antibodies
Description Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as aposttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composedof HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated.However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 orPro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degradedin proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIFdegradation and increases transcriptional activity. Therefore, the concentration of HIF-1alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition.HIF hydroxylases provide insight into hypoxic cell responses, which may be used to helpisolate therapeutic targets. Synonyms: Hypoxia-inducible factor 1 alpha antibody, HIF1 alpha antibody, ARNT interactingprotein antibody , Hif1a antibody, ARNT interacting protein antibody, HIF-1alphaantibody, Hypoxia inducible factor 1 alpha antibody, Hypoxia inducible factor 1 alphasubunit basic helix
Clone H1alpha67
Host Cell Max
Specificity This antibody is specific for HIF-1alpha. Recognizes human, sheep, mouse, rat, pig, bovine and ferret HIF-1 alpha.

Application Details:  HIF-1a (Bovine) antibody

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Application Immunohistochemistry (Frozen Sections) (IHC) (fro), Immunohistochemistry (paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunoblotting (IB)
Application Notes By Western blot, recognizes bands at 120kDa representing HIF-1 alpha in inducedtissues and cells. Multiple bands may be seen at 120kDa representing post-translationalmodification. Can also be used for immunohistochemical detection of humanHIF-1alpha.Suggested working dilutions: * Western Blot (ECL) 1:500-1,000** Immunohistochemistry 1:500-1,000Immunoprecipitation - not determined*The investigator should determine the optimal working dilution for a specific application.** Nuclear Extracts.
Format Purified
Concentration 3 mg/ml
Buffer 0.1 ml of Protein G purified Cell Max culture supernatant in PBS and 0.05% sodium PBS with 0.05% sodium azide
Storage Store at 4 degrees C. Avoid repeated freeze-thaw cycles.
Research Area Transcription Factors
Restrictions For Research Use only

External Information:  HIF-1a (Bovine) antibody

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Google Scholar Find more references for clone H1alpha67 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen Hypoxia-inducible Factor 1 alpha (HIF-1 alpha) (Bioinformatic Harvester (EMBL))