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Product details for Pancreatic Islet Lysate (Pig (Porcine)) antibody
Pancreatic Islet Lysate (Pig (Porcine)) antibody
Overview
| Antigen: | Pancreatic Islet Lysate |
| Antibody Type: | Polyclonal |
| Application: | Enzyme Immunoassay (EIA), Western Blotting (WB) |
| Reactivity: | Pig (Porcine) |
| Host: | Dog |
| Quantity: | |
| Price: | 400,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN116793 |
| Availability: | Ships in 3 to 10 days |
Additional Information: Pancreatic Islet Lysate (Pig (Porcine)) antibody
| Antigen | Pancreatic Islet Lysate |
| Immunogen | Sonicated crude membrane fraction of porcine pancreatic islets of langerhans cells. |
| Reactivity | Pig (Porcine) |
| Antibody Type | Polyclonal |
| Format | Ig Fraction |
| Host | Dog |
| Specificity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog Serum. Cross reactivity was determined by immunoblot (western) to be minimal against porcine kidney and porcine liver cell lysates. |
Application Details: Pancreatic Islet Lysate (Pig (Porcine)) antibody
| Application | Enzyme Immunoassay (EIA), Western Blotting (WB) |
| Application Notes | Suitable for immunoblotting (western or dot blot) and ELISA. This product has been assayed against 1.0 ug of Pancreatic Islet Lysate [Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&,L] (Goat) and o-Phenylenediamine (OPD) as a substrate. A working dilution of 1:50 to 1:100 is suggested for this product. This product has been assayed against 1.5 ug of Pancreatic Islet Lysate [Porcine Pancreas] per lane by immunoblot (western) under reducing conditions of SDS-PAGE prior to transfer to nitrocellulose and detection using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&,L] (Goat) and Diaminobenzidine (DAB) as a substrate. A working dilution of 1:50 to 1:200 is suggested for this product. Optimal titers for other applications should be determined by the researcher. Other applications not tested. |
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Buffer | This antibody is supplied as liquid, purified immunoglobulin fraction in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative. |
| Storage | Store vial at -20° C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening. 1 |
| Research Area | Cell/Tissue Markers |
| Restrictions | Research only |
Publications: Pancreatic Islet Lysate (Pig (Porcine)) antibody
| Publications |
Lanza, Jackson, Sullivan et al.: "Xenotransplantation of cells using biodegradable microcapsules." In: Transplantation , Vol. 67, Issue 8, pp. 1105-11, 1999/01 (PubMed) |
External Information: Pancreatic Islet Lysate (Pig (Porcine)) antibody
| Google Scholar | Find more references for antigen Pancreatic Islet Lysate on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Pancreatic Islet Lysate (Bioinformatic Harvester (EMBL)) |
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