LSP pSer252 (Human) antibody

Overview


	Antigen:	LSP pSer252
	Antibody Type:	Polyclonal
	Application:	Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
	Reactivity:	Human, Mouse (Murine), Rat
	Host:	Rabbit
	Quantity:	50 ug (0.5 mg/ml)
	Price:	250,00 € (Plus shipping costs and VAT)
	Order number:	ABIN123217
	Availability:	Ships in 3 to 10 days

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Additional Information: LSP pSer252 (Human) antibody

Antigen	LSP pSer252
Immunogen	A synthetic peptide corresponding to the carboxyl terminus of human LSP1 origin at the phosphorylated site of Serine 252.
Reactivity	Human, Mouse (Murine), Rat
Antibody Type	Polyclonal
Format	Aff - Purified
Host	Rabbit
Specificity	Leukocyte-specific protein 1 (LSP1, also known as Lymphocyte-specific protein1, SP34 and pp52) is a 52-kd filamentous-actin-binding intracellular phosphoprotein (1). Human LSP1 is composed of 339 amino acids, which expressed in normal and transformed B-lineage cells and normal T-lymphocytes (2-3). Studies show that LSP1 plays an important role in cytoskeletal architecture and motility regulation as well as receptor-induced apoptosis (4-6). Research show that LSP1 has at least three and potentially a fourth F-actin binding domain located in the basic C-terminal half (7). This F-actin binding sites are also correspond to the caldesmon and the villin headpiece proteins. LSP1 246-295 containing sequences homologous to caldesmon F-actin binding site II (CII) can bind F-actin. This l antibody reacts predominantly with LSP1. By immunoblot analysis, a ~52 kDa immunoreactive protein from stimulated Mouse Spleen cell lysate was detected (sample were boiled prior to subject 10% SDS-polyacrylamide gel electrophoresis). This band is abolished by the depletion of the synthetic phosphospecific peptide.

Application Details: LSP pSer252 (Human) antibody

Application	Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
Application Notes	ELISA (0.1 – 1.0 ug/ml), Western blot (0.5 – 2 ug/ml) and Immunoprecipitation (3.0 – 5.0 ug/extract from 106 cells). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration	0.5 mg/ml
Buffer	This antibody is supplied as liquid epitope affinity purified immunglobulin fraction in Phosphate buffered saline pH 7.4 with 0.02% Proclin300 as preservative.
Storage	Store the antibody undiluted at 4-8°C for one month or at –20°C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf life: one year from despatch.
Research Area	Phospho-specific antigens
Restrictions	Research only

Publications: LSP pSer252 (Human) antibody

Publications

Howard, Hartwig, Cunningham:
"Lymphocyte-specific protein 1 expression in eukaryotic cells reproduces the morphologic and motile abnormality of NAD 47/89 neutrophils."
In: Blood , Vol. 91, Issue 12, pp. 4786-95, 1998/01 (PubMed)

Klein, Galea, Jongstra:
"The lymphocyte-specific protein LSP1 is associated with the cytoskeleton and co-caps with membrane IgM."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 145, Issue 9, pp. 2967-73, 1990/01 (PubMed)

Shi, Zhang, Zhang et al.:
"Expression, purification, and activity identification of Alfimeprase in Pichia pastoris."
In: Protein expression and purification , Vol. 54, Issue 2, pp. 240-6, 2007/01 (PubMed)

Images: LSP pSer252 (Human) antibody

Western blot analysis of LSP1 protein on the stimulated Mouse Spleen cell lysate by anti-LSP1 (pSer252) at 1:500 (A) and depleted by Phosphoserine 252 peptide (SP6338CP) (B)

Image for LSP pSer252 (Human) antibody (1)

External Information: LSP pSer252 (Human) antibody

Google Scholar	Find more references for antigen LSP pSer252 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen LSP pSer252 (Bioinformatic Harvester (EMBL))