Ready-to-use Cell Proliferation Colorimetric Reagent, WST-1

Details for Product No. ABIN1304384, Supplier: Log in to see
Reactivity
Mammalian
Application
Detection (D), Proliferation Assay (ProA)
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Purpose The ready-to-use cell proliferation reagent, WST-1 provides a simple and accurate method to measure cell proliferation, which is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. Expansion in the number of viable cells results in an increase in the activity of the mitochondrial dehydrogenases, which in turn leads to increase in the amount of formazan dye formed. The formazan dye produced by viable cells can be quantified by measuring the absorbance at 440 nm. This new method is non-radioactive, rapid and more sensitive than MTT, XTT, or MTS-based assays. The entire assay can be performed in the same microtiter plate and does not require extra steps like washing, harvesting and cell solubilization.
Sample Type Cell Culture Cells, Biological Fluids, Adherent Cell Culture
Detection Method Colorimetric
Characteristics Ready-to-Use Cell Proliferation Reagent, WST-1: Measure Cell Proliferation in response to growth factors, cytokines, mitogens and nutrients etc. in the same microtiter plate. No need to wash, harvest & solubilize cells.
Simple procedure, Fast and convenient, The assay is non-radioactive, rapid and more sensitive than MTT, XTT, or MTS-based assays., The entire assay can be performed in the same microtiter plate and does not require extra steps like washing, harvesting and cell solubilization.

Detection wavelength: 440 nm. The ready-to-use cell proliferation reagent, WST-1 provides a simple and accurate method to measure cell proliferation, which is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. Expansion in the number of viable cells results in an increase in the activity of the mitochondrial dehydrogenases, which in turn leads to increase in the amount of formazan dye formed. The formazan dye produced by viable cells can be quantified by measuring the absorbance at 440 nm. This new method is non-radioactive, rapid and more sensitive than MTT, XTT, or MTS-based assays. The entire assay can be performed in the same microtiter plate and does not require extra steps like washing, harvesting and cell solubilization.
Components WST-1 Reagent (in electron coupling solution)
Background The ready-to-use cell proliferation reagent, WST-1 provides a simple and accurate method to measure cell proliferation, which is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. Expansion in the number of viable cells results in an increase in the activity of the mitochondrial dehydrogenases, which in turn leads to increase in the amount of formazan dye formed. The formazan dye produced by viable cells can be quantified by measuring the absorbance at 440 nm. This new method is non-radioactive, rapid and more sensitive than MTT, XTT, or MTS-based assays. The entire assay can be performed in the same microtiter plate and does not require extra steps like washing, harvesting and cell solubilization.
Application Notes Measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. Assessment of physiological mediators and antibodies that inhibit cell growth.
Comment

Further details regarding sample type: Cell cultures (adherent and suspension)

Restrictions For Research Use only
Storage -20 °C
Expiry Date 12 months
Supplier Images
 image for Ready-to-use Cell Proliferation Colorimetric Reagent, WST-1 (ABIN1304384) Ready-to-use Cell Proliferation Colorimetric Reagent, WST-1
Product cited in: Cheng, Chang, Chen, Chen: "Autophagy modulates endoplasmic reticulum stress-induced cell death in podocytes: a protective role." in: Experimental biology and medicine (Maywood, N.J.), Vol. 240, Issue 4, pp. 467-76, 2015 (PubMed).