Citrate Buffer pH 6.0

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Application
Immunohistochemistry (IHC)
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Characteristics Citrate Buffer, after dilution, is used on formalin-fixed, paraffin-embedded tissue sections mounted on glass slides for target retrieval prior to immunohistochemistry (IHC) procedures. When performed using this reagent and elevated temperature, target retrieval can disrupt the covalent bonds formed by formalin in tissue. Removal of these bonds can allow renaturation of protein molecules and increased antibody accessibility, which in turn can result in increased antibody binding (and thus increased staining intensity) and improved signal to noise ratios. This type of result is observed with many primary antibodies.
Protocol 1. Deparaffinize and rehydrate tissue sections.
2. Dilute the Citrate Buffer 1:10 with deionized water. Example: 20 ml of concentrate diluted in 180 ml of deionized water.
3. Immerse sections in coplin jar filled with the diluted Citrate Buffer reagent. Microwave on HIGH power until boiling.
4. Keep the slides warm by heating for 10 minutes on LOW power.
5. Let the coplin jar sit in microwave for at least 20 minutes.
6. Remove and rinse with water.
7. Rinse with buffer and proceed with the appropriate staining protocol for the primary antibody in use.
Restrictions For Research Use only
Format Liquid
Concentration 10 X
Buffer Citrate Buffer, pH 6.0. 10x concentrate.
Precaution of Use 1. Take reasonable precautions when handling reagents. Wear appropriate Personal Protective Equipment.
2. Avoid contact of reagents with eyes, mucous membranes and skin. If reagents come into contact with sensitive areas, wash with copious amounts of water.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
Storage RT
Storage Comment The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date.