Cell Proliferation Assay Reagent

Details for Product No. ABIN2344921, Supplier: Log in to see
Reactivity
Bacteria, Yeast, Fungi (Fusarium), Protozoa, Fish
Application
Proliferation Assay (ProA)
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Brand CytoSelect™
Sample Type Tissue Samples, Cell Extracts, Cell Samples
Detection Method Fluorometric
Characteristics The measurement and monitoring of cell proliferation is an essential technique in any laboratory focused on cell-based research. This skill allows for the optimization of cell culture conditions as well as the determination of cytokine, growth factor, or hormone activity. More importantly, the cytostatic nature of anticancer compounds in toxicology testing, the efficacy of therapeutic chemicals in drug screening, and cell-mediated cytotoxicity can all be assessed through the quantification and monitoring of cell proliferation. Cell proliferation characteristics include cellular metabolic activity and cell membrane integrity. One method for measuring metabolic activity is to incubate the cells with a tetrazolium salt such as MTT, which is cleaved into a colored formazan product by metabolically active cells. Similarly, the green fluorescent dye Calcein AM can measure intracellular esterase activity in proliferating live cells, which is another indicator of cell viability.
Material not included
  1. Cells for measuring proliferation
  2. Cell culture medium
  3. 24-well or 96-well clear or black-walled fluorescence microtiter cell culture plates. 2
  4. Fluorescence plate reader capable measuring fluorescence at 560 nm excitation wavelength and 590 nm emission wavelength.
Application Notes Optimal working dilution should be determined by the investigator.
Comment

  • Contains sufficient reagents for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates
  • Can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues
  • Cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells

Protocol CytoSelect™ Cell Proliferation Assay Reagent (Fluorometric) provides a fluorometric format for measuring and monitoring cell proliferation. The kit contains sufficient reagents for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates. Cells can be plated and then treated with compounds or agents that affect proliferation. Cells are then incubated with the proliferation reagent. Upon entering metabolically active live cells, the non-fluorescent proliferation reagent is converted into a bright red fluorescent form. An increase in cell proliferation is accompanied by increased fluorescent signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions. The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues. This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.
Assay Procedure
  1. Prepare a cell suspension containing 0.1-1.0 x 106 cells/mL in medium.
  2. Add 100 μL of cell suspension per well to a 96-well cell culture plate or 500 μL per well to a 24- well cell culture plate with or without the compound to be tested. Culture the cells for 24-96 hours at 37 °C and 5 % CO2 in a humidified incubator.
  3. Add one tenth volume of the CytoSelect™ Cell Proliferation Assay Reagent per well (for example add 10 μL of CytoSelect™ Cell Proliferation Assay Reagent per 100 μL of cell culture).
  4. Incubate plate at 37 °C and 5 % CO2 for 4-8 hours.
  5. Read the fluorescence with a fluorescence plate reader at 560 nm excitation wavelength and 590- 600 nm emission wavelength.
Restrictions For Research Use only
Format Liquid
Storage 4 °C
Storage Comment Store at 4°C and protect from light.
Supplier Images
Cellular Assay (CA) image for Cell Proliferation Assay Reagent (ABIN2344921) Human HEK 293 Cell Density. HEK 293 cells were seeded in triplicate at various densit...