7-AAD Viability Staining Solution

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Application
Multiplex Assay (MA)
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Characteristics 7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. When excited by 488 laser light, 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).
Components 7-AAD Viability Staining Solution
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 50 μg/mL
Buffer PBS, pH 7.2 with 0.09 % (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light.
Storage 4 °C
Storage Comment Store between 2°C and 8°C.
Background publications Ermann, Staton, Glickman, de Waal Malefyt, Glimcher: "Nod/Ripk2 signaling in dendritic cells activates IL-17A-secreting innate lymphoid cells and drives colitis in T-bet-/-.Rag2-/- (TRUC) mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 25, pp. E2559-66, 2014 (PubMed).

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Wedel, Hottenrott, Stamellou, Breedijk, Tsagogiorgas, Hillebrands, Yard: "N-Octanoyl dopamine transiently inhibits T cell proliferation via G1 cell-cycle arrest and inhibition of redox-dependent transcription factors." in: Journal of leukocyte biology, Vol. 96, Issue 3, pp. 453-62, 2014 (PubMed).

Jaworska, Ratajczak, Huang, Whalen, Yang, Stevens, Kinsey: "Both PD-1 ligands protect the kidney from ischemia reperfusion injury." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 1, pp. 325-33, 2014 (PubMed).

Kong, Li, Tuma, Ganea: "Selective CB2 receptor activation ameliorates EAE by reducing Th17 differentiation and immune cell accumulation in the CNS." in: Cellular immunology, Vol. 287, Issue 1, pp. 1-17, 2014 (PubMed).

Kraaij, Vereyken, Leenen, van den Bosch, Rezaee, Betjes, Baan, Rowshani: "Human monocytes produce interferon-gamma upon stimulation with LPS." in: Cytokine, Vol. 67, Issue 1, pp. 7-12, 2014 (PubMed).

Schneider, Setty, Holmes, Maute, Leslie, Mussolin, Rosolen, Dalla-Favera, Basso: "MicroRNA 28 controls cell proliferation and is down-regulated in B-cell lymphomas." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 22, pp. 8185-90, 2014 (PubMed).

Harker, Ueno, Wang, Bonhomme, Liu, Lodoen: "Toxoplasma gondii modulates the dynamics of human monocyte adhesion to vascular endothelium under fluidic shear stress." in: Journal of leukocyte biology, Vol. 93, Issue 5, pp. 789-800, 2013 (PubMed).

Mikhak, Strassner, Luster: "Lung dendritic cells imprint T cell lung homing and promote lung immunity through the chemokine receptor CCR4." in: The Journal of experimental medicine, Vol. 210, Issue 9, pp. 1855-69, 2013 (PubMed).

Wu, Lu, Antony, Juhasz, Liu, Jiang, Meitzler, Hollingshead, Haines, Butcher, Roy, Doroshow: "Activation of TLR4 is required for the synergistic induction of dual oxidase 2 and dual oxidase A2 by IFN-? and lipopolysaccharide in human pancreatic cancer cell lines." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 190, Issue 4, pp. 1859-72, 2013 (PubMed).

Zhang, Xu, Fu, Liu, Jiang, Cui, Zhao, Sun, He, Li, Shang: "Transcriptomic analysis of peripheral blood mononuclear cells in rapid progressors in early HIV infection identifies a signature closely correlated with disease progression." in: Clinical chemistry, Vol. 59, Issue 8, pp. 1175-86, 2013 (PubMed).

Cohen, Briggs, Marino, Ravid, Robson, Mosser: "TLR stimulation initiates a CD39-based autoregulatory mechanism that limits macrophage inflammatory responses." in: Blood, Vol. 122, Issue 11, pp. 1935-45, 2013 (PubMed).

Hufbauer, Biddle, Borgogna, Gariglio, Doorbar, Storey, Pfister, Mackenzie, Akgül: "Expression of betapapillomavirus oncogenes increases the number of keratinocytes with stem cell-like properties." in: Journal of virology, Vol. 87, Issue 22, pp. 12158-65, 2013 (PubMed).

Teng, Mercer, Hexley, Thomas, Fumagalli: "Loss of tumor suppressor RPL5/RPL11 does not induce cell cycle arrest but impedes proliferation due to reduced ribosome content and translation capacity." in: Molecular and cellular biology, Vol. 33, Issue 23, pp. 4660-71, 2013 (PubMed).

Hussain, Baig, Neumann, Peche, Szczepanski, Nürnberg, Tariq, Jameel, Khan, Fatima, Malik, Ahmad, Altmüller, Frommolt, Thiele, Höhne, Yigit, Wollnik, Neubauer, Nürnberg, Noegel: "CDK6 associates with the centrosome during mitosis and is mutated in a large Pakistani family with primary microcephaly." in: Human molecular genetics, Vol. 22, Issue 25, pp. 5199-214, 2013 (PubMed).

Turner, Morrison, Wilhelm, Wilson, Ahlfors, Renauld, Panzer, Helmby, Stockinger: "IL-9-mediated survival of type 2 innate lymphoid cells promotes damage control in helminth-induced lung inflammation." in: The Journal of experimental medicine, Vol. 210, Issue 13, pp. 2951-65, 2013 (PubMed).

Choudhury, Raychaudhuri, Bhattacharyya: "Identification of HYPK-interacting proteins reveals involvement of HYPK in regulating cell growth, cell cycle, unfolded protein response and cell death." in: PLoS ONE, Vol. 7, Issue 12, pp. e51415, 2012 (PubMed).

Sandhu, Volinia, Costinean, Galasso, Neinast, Santhanam, Parthun, Perrotti, Marcucci, Garzon, Croce: "miR-155 targets histone deacetylase 4 (HDAC4) and impairs transcriptional activity of B-cell lymphoma 6 (BCL6) in the E?-miR-155 transgenic mouse model." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 49, pp. 20047-52, 2012 (PubMed).

Wigren, Björkbacka, Andersson, Ljungcrantz, Fredrikson, Persson, Bryngelsson, Hedblad, Nilsson: "Low levels of circulating CD4+FoxP3+ T cells are associated with an increased risk for development of myocardial infarction but not for stroke." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 32, Issue 8, pp. 2000-4, 2012 (PubMed).