Permeabilization Wash Buffer (10X)

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Application
Multiplex Assay (MA)
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Characteristics Permeabilization Wash Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Permeabilization Wash Buffer is used to permeabilize cells following fixation with Fixation Buffer (Cat. No. 420801). It is supplied as a 10X solution and should be diluted in deionized water prior to use. Permeabilization Wash Buffer has been formulated to have minimal effects on cells, reduce non-specific staining and enhance the signal to noise ratio. It can be used for antibody dilutions and cell washing during intracellular staining.
Components Permeabilization Wash Buffer (10X)
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 10 X
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment This permeabilization wash buffer solution should be stored between 2°C and 8°C.
Background publications Kusner, Ciesielski, Marx, Kaminski, Fenstermaker: "Survivin as a potential mediator to support autoreactive cell survival in myasthenia gravis: a human and animal model study." in: PLoS ONE, Vol. 9, Issue 7, pp. e102231, 2014 (PubMed).

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Marongiu, Donini, Toffali, Zenaro, Dusi: "ESAT-6 and HspX improve the effectiveness of BCG to induce human dendritic cells-dependent Th1 and NK cells activation." in: PLoS ONE, Vol. 8, Issue 10, pp. e75684, 2013 (PubMed).

del Rio, Jones, Buhler, Norris, Shintani, Ware, Rodriguez-Barbosa: "Selective blockade of herpesvirus entry mediator-B and T lymphocyte attenuator pathway ameliorates acute graft-versus-host reaction." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 10, pp. 4885-96, 2012 (PubMed).

del Rio, Kurtz, Perez-Martinez, Ghosh, Perez-Simon, Rodriguez-Barbosa: "B- and T-lymphocyte attenuator targeting protects against the acute phase of graft versus host reaction by inhibiting donor anti-host cytotoxicity." in: Transplantation, Vol. 92, Issue 10, pp. 1085-93, 2011 (PubMed).

Smeltz: "Profound enhancement of the IL-12/IL-18 pathway of IFN-gamma secretion in human CD8+ memory T cell subsets via IL-15." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 8, pp. 4786-92, 2007 (PubMed).

Kang, Kim, Shimada, Otsuka, Seit-Nebi, Kwon, Watts, Han: "Cell surface 4-1BBL mediates sequential signaling pathways 'downstream' of TLR and is required for sustained TNF production in macrophages." in: Nature immunology, Vol. 8, Issue 6, pp. 601-9, 2007 (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996 (PubMed).

Sander, Höidén, Andersson, Möller, Abrams: "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." in: Journal of immunological methods, Vol. 166, Issue 2, pp. 201-14, 1994 (PubMed).

Sander, Andersson, Andersson: "Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure." in: Immunological reviews, Vol. 119, pp. 65-93, 1991 (PubMed).