Human BD Fc Block™

Details for Product No. ABIN2690968, Supplier: Log in to see
Reactivity
Human, Rhesus Monkey
Application
Flow Cytometry (FACS)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Brand BD Pharmingen™
Characteristics Fcγ Receptors belong to the immunoglobulin superfamily and are expressed at varying levels in multiple cell lineages including high expression in myeloid and B cells. The major functions of Fc receptors are protective functions of the immune system. There are multiple different types of Fc receptors reflecting a variety of different biological activities, which are modulated when they are aggregated by multivalent antigen-antibody complexes. While normally serving important physiological roles in the immune system, Fc Receptors can also be the cause of nonspecific, false-positive antibody staining of cells. Human BD Fc Block™ is designed and formulated to block or significantly reduce potential non-specific antibody staining caused by receptors for IgG that may be encountered in various applications including the flow cytometric analysis of human cells. Moreover, it can increase the specificity of antibody labeling of extremely rare target cells such as antigen-specific B cells, fetal cells in maternal blood, hematopoietic progenitor cells, or disseminated epithelial tumor cells.
Sterility Sterile filtered,0.2 μm filtered
Endotoxin Level Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay.
Application Notes Optimal working dilution should be determined by the investigator.
Comment

BD Pharmingen™ Human BD Fc Block™ - Purified NA/LE Reactivity Hu, Rhe

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Product cited in: Nimmerjahn, Ravetch: "Fc-receptors as regulators of immunity." in: Advances in immunology, Vol. 96, pp. 179-204, 2007 (PubMed).

Ravetch, Bolland: "IgG Fc receptors." in: Annual review of immunology, Vol. 19, pp. 275-90, 2001 (PubMed).