(2 references)-
- Reactivity
- Human, Rhesus Monkey
- Application
- Flow Cytometry (FACS)
- Brand
- BD Pharmingen™
- Characteristics
- Fcγ Receptors belong to the immunoglobulin superfamily and are expressed at varying levels in multiple cell lineages including high expression in myeloid and B cells. The major functions of Fc receptors are protective functions of the immune system. There are multiple different types of Fc receptors reflecting a variety of different biological activities, which are modulated when they are aggregated by multivalent antigen-antibody complexes. While normally serving important physiological roles in the immune system, Fc Receptors can also be the cause of nonspecific, false-positive antibody staining of cells. Human BD Fc Block™ is designed and formulated to block or significantly reduce potential non-specific antibody staining caused by receptors for IgG that may be encountered in various applications including the flow cytometric analysis of human cells. Moreover, it can increase the specificity of antibody labeling of extremely rare target cells such as antigen-specific B cells, fetal cells in maternal blood, hematopoietic progenitor cells, or disseminated epithelial tumor cells.
- Sterility
- Sterile filtered,0.2 μm filtered
- Endotoxin Level
- Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay.
-
Blocking Buffer for Fluorescent Western Blotting
BR, Lbl, WB 1 X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
ELISA Coating StabilizerELISA 1 X
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
BD Pharmingen™ Human BD Fc Block™ - Purified NA/LE Reactivity Hu, Rhe
- Restrictions
- For Research Use only
-
- Concentration
- 1 mg/mL
- Buffer
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
- Preservative
- Azide free
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
-
-
You are here:
