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Annexin V Binding Buffer (1X)

Details for Product No. ABIN412353, Supplier: Log in to see
Application
Apoptosis Detection (AD)
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Characteristics Buffer for use with the multi-color bulk annexin V reagents including annexin V-FITC (Cat# 1001-1000), annexin V-EGFP (Cat.# 1004-1000), annexin V-Cy3 (Cat.# 1002-1000), annexin V-Cy5 (1013-1000), annexin V-Biotin (Cat.# 1003-100), annexin V-PE (1014-1000) and unlabeled annexin V (1005-100). During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore serves as a probe for detecting apoptosis. 1X Annexin V Binding Buffer is ready to use for annexin V binding assay to detect PS exposure during apoptosis.
Comment

Buffer for use with the multi-color bulk annexin V reagents including annexin V-FITC is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore serves as a probe for detecting apoptosis. 1X Annexin V Binding Buffer is ready to use for annexin V binding assay to detect PS exposure during apoptosis.

Restrictions For Research Use only
Format Liquid
Concentration 1 X
Storage 4 °C
Expiry Date 12 months
Product cited in: Mirza, Teo, Upcroft, Tan: "A rapid, high-throughput viability assay for Blastocystis spp. reveals metronidazole resistance and extensive subtype-dependent variations in drug susceptibilities." in: Antimicrobial agents and chemotherapy, Vol. 55, Issue 2, pp. 637-48, 2011 (PubMed).

Janda, Visalli, Colica, Aprigliano, Musolino, Vadala: "The protective effect of tianeptine on Gp120-induced apoptosis in astroglial cells: role of GS and NOS, and NF-κB suppression." in: British journal of pharmacology, 2010 (PubMed).

Henderson, Shaw, Wang, Han, Hurley, Flynn, Dorr, Von Hoff: "UA62784, a novel inhibitor of centromere protein E kinesin-like protein." in: Molecular cancer therapeutics, Vol. 8, Issue 1, pp. 36-44, 2009 (PubMed).

Kim, Shu, Xu, Shiau, Grant, Vasile, Cosford, Reed: "Chemical biology investigation of cell death pathways activated by endoplasmic reticulum stress reveals cytoprotective modulators of ASK1." in: The Journal of biological chemistry, Vol. 284, Issue 3, pp. 1593-603, 2009 (PubMed).

Guida, Bisso, Fenollar-Ferrer, Napoli, Anselmi, Girardini, Carloni, Del Sal: "Peptide aptamers targeting mutant p53 induce apoptosis in tumor cells." in: Cancer research, Vol. 68, Issue 16, pp. 6550-8, 2008 (PubMed).

Xi, Dyer, Kimak, Zhang, Gooding, Chaillet, Chai, Ferrell, Zamboni, Hunt, Grandis: "Decreased STAT1 expression by promoter methylation in squamous cell carcinogenesis." in: Journal of the National Cancer Institute, Vol. 98, Issue 3, pp. 181-9, 2006 (PubMed).