Images for product: ECL substrate (Mark-It Glow liquid)
Western Blot developed with Mark-It Glow Liquid ECL substrate. Decreasing amounts of a control protein were subjected to Western Blotting (Lanes 1-7: 300ng, 100 ng, 30 ng, 10 ng, 3 ng 1 ng, 300 pg). The blocked membrane was probed with a specific primary antibody and bound antibodies were detected with a specific HRP conjugate. Visualization was performed using MicroMol’s Mark-It Glow Liquid ECL substrate.
ECL substrate Mark-It Glow Liquid is superior to its competitors on the market. Mark-It Glow Liquid as well as one of the leading ECL substrates on the market were activated by horseradish peroxidase (0.05 nU/ml) and light emission was measured luminometric at the indicated points of time.