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TMB ELISA Peroxidase Substrate

ELISA 1 X
Catalog No. ABIN925629
  • Application
    ELISA
    Characteristics
    TMB ELISA Peroxidase Substrate (3, 3', 5, 5' - Tetramethylbenzidine) is a chromogenic substrate used to visualize antibody reactivity in ELISA experiments. In the presence of peroxidases, TMB can act as an electron donor for the conversion of peroxides to water, changing the color of solution to a blue color equivalent to the degree of reactivity. This reaction can be halted with acid to change the TMB to yellow.
    Synonyms: 3,3’,5,5’-Tetramethylbenzidine; TMB-E; TMBE
  • Application Notes
    TMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay conditions. No dilutions are required, TMB ELISA Peroxidase Substrate comes ready to use.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 X
    Storage
    4 °C
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