anti-Human Tumor Necrosis Factor antibody for Flow Cytometry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Reactivity
Human
1250
461
359
145
136
119
119
63
55
41
29
18
14
12
9
7
6
4
4
4
4
2
1
1
1
1
Host
Mouse
934
519
184
48
10
9
7
6
4
3
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is conjugated to PE
169
100
73
50
32
30
28
27
26
25
25
25
25
25
24
24
24
23
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS)
887
858
621
321
287
230
219
190
138
118
97
69
29
29
26
22
20
18
14
9
7
7
6
6
6
5
5
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN1383957
$ 326.87
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.327583 ABIN1383956 FACS FITC Mouse IgG1 Log in to see B-D9 1
14.327583 ABIN1997163 CC ELISA FACS Mouse IgG1 AA 77-233 Log in to see 9A7A5G9 1
14.327583 ABIN263068 ELISA FACS ICC IF IHC IHC (fro) IHC (p) Mouse IgG1 kappa Log in to see MAb11 1
14.327583 ABIN263070 ICC FACS IF IHC (p) FITC Mouse IgG1 kappa Log in to see MAb11 1
14.327583 ABIN4898910 FACS PE Mouse IgG1 Log in to see 6402 7
14.327583 ABIN4898909 FACS Fluorescein Mouse IgG1 Log in to see 6402 4
14.327583 ABIN2658839 FACS APC Mouse IgG1 kappa Log in to see MAb11 10
14.327583 ABIN2662374 FACS Pacific Blue Mouse IgG1 kappa Log in to see MAb11 11
14.327583 ABIN2659787 BR FACS IF IHC PE/Dazzle™ 594 Mouse IgG1 kappa Log in to see MAb11 10
14.327583 ABIN2659610 FACS PE-Cy7 Mouse IgG1 kappa Log in to see MAb11 10
14.327583 ABIN2657587 FACS Alexa Fluor 488 Mouse IgG1 kappa Log in to see MAb11 10
14.327583 ABIN2660503 FACS PerCP-Cy5.5 Mouse IgG1 kappa Log in to see MAb11 10
14.327583 ABIN2662036 FACS FITC Mouse IgG1 kappa Log in to see MAb11 11
14.327583 ABIN2657286 FACS Alexa Fluor 700 Mouse IgG1 kappa Log in to see MAb11 10
14.327583 ABIN2660627 FACS APC-Cy7 Mouse IgG1 kappa Log in to see MAb11 10
14.327583 ABIN2663940 FACS PE Mouse IgG1 kappa Log in to see MAb11 10
14.327583 ABIN1997166 FACS FITC Mouse IgG1 AA 77-233 Log in to see 01
14.327583 ABIN5503749 FACS Fluorescein Mouse IgG1 AA 57-233, Membrane Form Log in to see 6401
14.327583 ABIN5503750 FACS PE Mouse IgG1 AA 57-233, Membrane Form Log in to see 6401
14.327583 ABIN5503748 FACS Fluorescein Mouse IgG1 AA 57-233, Membrane Form Log in to see 6401

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human
(1250), (461), (359), (145), (136), (119), (119), (63), (55), (41), (29), (18), (14), (12), (9), (7), (6), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Host Mouse
(934), (519), (184), (48), (10), (9), (7), (6), (4), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is conjugated to PE
(169), (100), (73), (50), (32), (30), (28), (27), (26), (25), (25), (25), (25), (25), (24), (24), (24), (23), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS)
(887), (858), (621), (321), (287), (230), (219), (190), (138), (118), (97), (69), (29), (29), (26), (22), (20), (18), (14), (9), (7), (7), (6), (6), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN1383957) Images
Specificity Recognises both natural and recombinant TNF-a
Characteristics Recombinant human TNF-a.
Purification Ion exchange chromatography
Clone B-D9
Isotype IgG1

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN1383957) Images back to top
Antigen
Alternative Name TNF-a (TNF Antibody Abstract)
Background Myeloma X63/AG.8653 x Balb/c spleen cells
Research Area Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN1383957) Images back to top
Application Notes Suitable for intra-cytoplasmic flow cytometry
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN1383957) Images back to top
Format Liquid
Reconstitution Reconstitute with 1 mL deionized water
Buffer Phosphate-buffered saline with 5 % BSA and 0,1 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice It is recommended to establish the optimal dilution according to the system being used. Use 10 µL of (diluted) antibody to label 5 x 10 5 cells. Do not freeze.
Storage 4 °C
Storage Comment Declared stable after 7 days at room temperature, 6 months at +2-8°C after reconstitution. Protect from light. Do not freeze.
Expiry Date 6 months

ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN1383957)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Arduise, Abache, Li, Billard, Chabanon, Ludwig, Mauduit, Boucheix, Rubinstein, Le Naour: "Tetraspanins regulate ADAM10-mediated cleavage of TNF-alpha and epidermal growth factor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 10, pp. 7002-13, 2008

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN1383957) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor (TNF) antibody (PE) (ABIN1383957) A typical staining pattern with the B-D9 monoclonal antibody of PMA and ionomycin act...